Gene Rv0697
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Probable dehydrogenase |
| Comments | Rv0697, (MTCY210.14, unknown), len: 479 aa. Probable dehydrogenase, highly similar to P30772|YTUR_MYCLE hypothetical 24 kDa protein from Mycobacterium leprae (220 aa), FASTA scores: opt: 557, E(): 1.7e-28, (46.2% identity in 223 aa overlap). Also highly similar to P46371|YTH2_RHOER hypothetical 53.0 KDA GMC-type oxidoreductase from Rhodococcus erythropolis (493 aa); and similar to many dehydrogenases e.g. NP_250814.1|NC_002516 probable dehydrogenase from Pseudomonas aeruginosa (545 aa); BAA13145.1|D86622 FAD dependent L-sorbose dehydrogenase from Gluconobacter oxydans (531 aa); etc. Also similar to Rv1279 conserved hypothetical protein from Mycobacterium tuberculosis. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 796933 | 798372 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0697|Rv0697
VTAAVRHSDVLVVGAGSAGSVVAERLSMDSSCVVTVLEAGPGLADPGLLAQTANGLQLPIGAGSPLVERYRTRLTDRPVRHLPIVRGATVGGSGAINGGYFCRGLPSDFDRASIPGWAWSDVLEHFRAIETDLDFETPVHGRSGPIPVRRTHEMTGITESFMAAAEDAGFAWIADLNDVGPEMPSGVGAVPLNIVNGVRTSSAVGYLMPALGRPNLTLLARTRAVRLRFSATTAVGVDAIGPGGPVSLSADRIVLCAGAIQSAHLLMLSGVGEEEVLRSAGVKVLMALPVGMGCSDHPEWVMPTNWAVAVDRPVLEVLLSTHDGIEIRPYTGGFVAMTGDGTAGHRDWPHIGVALMQPRARGRITLVSSDPQIPVRIEHRYDSEPADVAALRQGSALAHELCGAATRIGPAVWATSQHLCGSAPMGTDDDPRAVVDPRCRVRGIENLWVIDGSVLPSITSRGPHATIVMLGHRAAEFVQ
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
