Gene Rv0840c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Specifically catalyzes the removal of N-terminal proline residues from peptides. Thought to release the N-terminal proline from the dipeptides, pro-pro, pro-GLN, pro-TRP and pro-TYR; also from amides (pro-beta NA) and oligopeptides, pro-LEU-GLYNH2, pro-LEU-GLY and pro-PHE-GLY-LYS. Higher activity toward small peptides (up to three residues), but very low activity for longer peptides [catalytic activity: release of a N-terminal proline from a peptide]. |
| Product | Probable proline iminopeptidase Pip (prolyl aminopeptidase) (pap) |
| Comments | Rv0840c, (MTV043.33c), len: 286 aa. Possible pip, proline iminopeptidase, similar to many e.g. P46541|PIP_BACCO proline iminopeptidase from bacillus coagulans (288 aa), FASTA scores: opt: 657, E(): 0, (37.6% identity in 282 aa overlap); NP_386922.1|NC_003047 putative proline iminopeptidase protein from Sinorhizobium meliloti (296 aa); etc. Belongs to peptidase family S33. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 936457 | 937317 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0840c|pip
VEGTIAVPGGRVWFQRIGGGPGRPLLVVHGGPGLPHNYLAPLRRLSDEREVIFWDQLGCGNSACPSDVDLWTMNRSVAEMATVAEALALTRFHIFSHSWGGMLAQQYVLDKAPDAVSLTIANSTASIPEFSASLVSLKSCLDVATRSAIDRHEAAGTTHSAEYQAAIRTWNETYLCRTRPWPRELTEAFANMGTEIFETMFGPSDFRIVGNVRDWDVVDRLADIAVPTLLVVGRFDECSPEHMREMQGRIAGSRLEFFESSSHMPFIEEPARFDRVMREFLRLHDI
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
