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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in tricarboxylic acid cycle (KREBS cycle) [catalytic activity: citrate + CoA = acetyl-CoA + H2O + oxaloacetate].
ProductProbable citrate synthase II CitA
CommentsRv0889c, (MTCY31.17c), len: 373 aa. Probable citA (alternate gene name: gltA), citrate synthase 2, highly similar to others e.g. CAB95899.1|AL359988 putative citrate synthase from Streptomyces coelicolor (387 aa); P39119|CISY_BACSU citrate synthase II from Bacillus subtilis (366 aa), FASTA scores: opt: 586, E(): 5.8e-30, (33.8% identity in 367 aa overlap); etc. Also similar to Rv0896|MTCY31.24 from Mycobacterium tuberculosis (29.2% identity in 274 aa overlap) and Rv1131. Contains PS00480 Citrate synthase signature. Belongs to the citrate synthase family.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS988740989861-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0889c|citA
MTVVPENFVPGLDGVVAFTTEIAEPDKDGGALRYRGVDIEDLVSQRVTFGDVWALLVDGNFGSGLPPAEPFPLPIHSGDVRVDVQAGLAMLAPIWGYAPLLDIDDATARQQLARASVMALSYVAQSARGIYQPAVPQRIIDECSTVTARFMTRWQGEPDPRHIEAIDAYWVSAAEHGMNASTFTARVIASTGADVAAALSGAIGAMSGPLHGGAPARVLPMLDEVERAGDARSVVKGILDRGEKLMGFGHRVYRAEDPRARVLRAAAERLGAPRYEVAVAVEQAALSELRERRPDRAIETNVEFWAAVVLDFARVPANMMPAMFTCGRTAGWCAHILEQKRLGKLVRPSAIYVGPGPRSPESVDGWERVLTTA