Gene Rv0908
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. |
| Product | Probable metal cation transporter ATPase P-type CtpE |
| Comments | Rv0908, (MTCY21C12.02), len: 797 aa. Probable ctpE, metal cation-transporting ATPase P-type, transmembrane protein, E1-E2 family, highly similar to many e.g. AB93406.1|AL357524 putative integral membrane ATPase from Streptomyces coelicolor (802 aa); NP_346063.1|NC_003028 cation-transporting ATPase (E1-E2 family) from Streptococcus pneumoniae (778 aa); P37278|ATCL_SYNP7|PACL cation-transporting atpase from Synechococcus sp. strain PCC 7942 (Anacystis nidulans R2) (926 aa), FASTA scores: opt: 257, E(): 4.8e-33, (27.7% identity in 905 aa overlap); etc. Contains E1-E2 ATPases phosphorylation site (PS00154). Belongs to the cation transport ATPases family (E1-E2 ATPases). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1011731 | 1014124 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0908|ctpE
MTRSASATAGLTDAEVAQRVAEGKSNDIPERVTRTVGQIVRANVFTRINAILGVLLLIVLATGSLINGMFGLLIIANSVIGMVQEIRAKQTLDKLAIIGQAKPLVRRQSGTRTRSTNEVVLDDIIELGPGDQVVVDGEVVEEENLEIDESLLTGEADPIAKDAGDTVMSGSFVVSGAGAYRATKVGSEAYAAKLAAEASKFTLVKSELRNGINRILQFITYLLVPAGLLTIYTQLFTTHVGWRESVLRMVGALVPMVPEGLVLMTSIAFAVGVVRLGQRQCLVQELPAIEGLARVDVVCADKTGTLTESGMRVCEVEELDGAGRQESVADVLAALAAADARPNASMQAIAEAFHSPPGWVVAANAPFKSATKWSGVSFRDHGNWVIGAPDVLLDPASVAARQAERIGAQGLRVLLLAAGSVAVDHAQAPGQVTPVALVVLEQKVRPDARETLDYFAVQNVSVKVISGDNAVSVGAVADRLGLHGEAMDARALPTGREELADTLDSYTSFGRVRPDQKRAIVHALQSHGHTVAMTGDGVNDVLALKDADIGVAMGSGSPASRAVAQIVLLNNRFATLPHVVGEGRRVIGNIERVANLFLTKTVYSVLLALLVGIECLIAIPLRRDPLLFPFQPIHVTIAAWFTIGIPAFILSLAPNNERAYPGFVRRVMTSAVPFGLVIGVATFVTYLAAYQGRYASWQEQEQASTAALITLLMTALWVLAVIARPYQWWRLALVLASGLAYVVIFSLPLAREKFLLDASNLATTSIALAVGVVGAATIEAMWWIRSRMLGVKPRVWR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
