Gene Rv0914c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in degradative pathways such as fatty acid BETA_OXIDATION. |
| Product | Possible lipid carrier protein or keto acyl-CoA thiolase |
| Comments | Rv0914c, (MTCY21C12.08c), len: 412 aa. Possible lipid carrier protein or keto acyl-CoA thiolase, highly similar to NP_421905.1|NC_002696 thiolase family protein from Caulobacter crescentus (407 aa); and similar to others e.g. NP_107896.1|NC_002678 3-ketoacyl-CoA thiolase from Mesorhizobium loti (392 aa); NP_385796.1|NC_003047 putative 3-ketoacyl-CoA thiolase protein from Sinorhizobium meliloti (389 aa); NP_275932.1|NC_000916 lipid-transfer protein (sterol or nonspecific) from Methanothermobacter thermautotrophicus (383 aa); AB55378.1|AL117263 possible 3-ketoacyl-CoA thiolase from Leishmania major (441 aa), FASTA scores: opt: 547, E(): 3.1e-26, (31.0% identity in 435 aa overlap); etc. Also similar to Rv2790c, Rv1627c, Rv0244, etc from Mycobacterium tuberculosis. Could belong to the thiolase family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1018727 | 1019965 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0914c|Rv0914c
MDDGVWILGGYQSDFARNLSKENRDFADLTREVVDGTLTAAKVDAADLAAAGVVHVANAFGEMFARQGHLGAMPATVCDDLWDTPATRHEAACASGSVATLAAMADLRSGAYRVALVVGLELEKTVPGDTAAEHLSAAAWTGHEGAEARYLWPSMFAQVADEYDRRYGLDDTHLRAIAQLNFANARRNPNAQTRGWTIPDPITDDDATNPLTEGRLRRFDCSQMTDGGAGLVLVSDAYLRDHRDARPIGRIDGWGHRTVGLGLRQKLDRVAQGDSAPYLLPHVRATVLDALRRARVTLDDLDGIEVHDCFTPSEYLAIDHIGLTGPGESWKAIENGEIEIGGRLPINPSGGLIGGGHPVGASGVRMLLDAAKQVSGIAGDYQVENAEAFGTLNFGGSTATTVSFVVSTTRGS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
