Gene Rv0986
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in active transport of adhesion component across the membrane: involved in attachment and virulence. Responsible for energy coupling to the transport system. |
| Product | Probable adhesion component transport ATP-binding protein ABC transporter |
| Comments | Rv0986, (MTV044.14), len: 248 aa. Probable ATP-binding protein ABC transporter supposedly involved in transport of adhesion component (see citation below), highly similar to many ATP-binding proteins e.g. AE0010|AE001033_8 ABC transporter ATP-binding protein from Archaeoglobus fulgidus (228 aa), FASTA scores: opt: 669, E(): 0, (45.7% identity in 219 aa overlap); CAB81857.1|AL161691 putative ABC-transporter ATP-binding protein from Streptomyces coelicolor (246 aa); X84019|ZMDNAGRP_4 glutamate uptake regulatory protein (grp) from Z.mobilis (232 aa), FASTA score: (44.4% identity in 225 aa overlap); Z99111|BSUB0008_108 from Bacillus subtilis (230 aa), FASTA score: (38.7% identity in 222 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00211 ABC transporters family signature. Belongs to the ATP-binding transport protein family (ABC transporters). Believed to have been acquired by horizontal gene transfer (See Rosas-Magallanes et el., 2006; Becq et al., 2007). |
| Functional category | Cell wall and cell processes |
| Transcriptomics | DNA microarrays detect expression in M. tuberculosis H37Rv in vivo (in BALB/c and SCID mice) but not in vitro (in 7H9 medium) (See Talaat et al., 2004). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis CDC1551 transposon mutant fails to prevent phagosome maturation and acidification in bone marrow macrophages from BALB/c mice; mutant shows reduced survival in these macrophages (See Pethe et al., 2004). M. tuberculosis CDC1551 transposon mutant is attenuated in the central nervous system of BALB/c mice (See Be et al., 2008). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1101803 | 1102549 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv0986|Rv0986
MNRQPIVQLSNLSWTFREGETRRQVLDHITFDFEPGEFVALLGQSGSGKSTLLNLISGIEKPTTGDVTINGFAITQKTERDRTLFRRDQIGIVFQFFNLIPTLTVLENITLPQELAGVSQRKAAVVARDLLEKVGMADRERTFPDKLSGGEQQRVAISRALAHNPMLVLADEPTGNLDSDTGDKVLDVLLDLTRQAGKTLIMATHSPSMTQHADRVVNLQGGRLIPAVNRENQTDQPASTILLPTSYE
Bibliography
- Braibant M et al. [2000]. The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. Review Secondary
- Pethe K et al. [2004]. Isolation of Mycobacterium tuberculosis mutants defective in the arrest of phagosome maturation. Mutant
- Talaat AM et al. [2004]. The temporal expression profile of Mycobacterium tuberculosis infection in mice. Transcriptome
- Rosas-Magallanes V et al. [2006]. Horizontal transfer of a virulence operon to the ancestor of Mycobacterium tuberculosis. Sequence
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Be NA et al. [2008]. Murine model to study the invasion and survival of Mycobacterium tuberculosis in the central nervous system. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
