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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionThought to be involved in detoxification reactions following oxidative damage to lipids [catalytic activity: an epoxide + H(2)O = a glycol].
ProductProbable epoxide hydrolase EphC (epoxide hydratase)
CommentsRv1124, (MTCY22G8.13), len: 316 aa. Probable ephC, epoxide hydrolase (see citation below), similar to Q42566 epoxide hydrolase from Arabidopsis thaliana (321 aa), FASTA scores: opt: 298, E(): 8.2e-13, (27.6% identity in 333 aa overlap). Similar to other M. tuberculosis epoxide hydrolases and non-heme bromoperoxidases e.g. Rv1938, Rv3617, Rv3670, Rv3473c, etc.
Functional categoryVirulence, detoxification, adaptation
ProteomicsIdentified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cytosol and cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the detergent phase of Triton X-114 extracts of M. tuberculosis H37Rv membranes using 1-DGE and MALDI-TOF-MS (See Sinha et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS12471271248077+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1124|ephC
MRAGRGERESTWRTTMAEPHWIDVKGPNGDLKALTWGPAGAPVALCLHGFPDTAYGWRKVAPRLAESGWHVVAPFMRGYAPSSIPADGSYHVGALMHDALRVRSAAGGTERDVIIGHDWGAIAATGLAAMPDSPFAKAVIMSVPPSAAFRPLGRVPERGRLLRELPHQLLRSWYILYFQLPWLPERSASWVVPLLWRRWSPGYHAEEDLRHVDAAIGTPEGRRAALGPYRATMRNTRAPADYADLNRLWTEAPKLPVLYLHGHDDGCATSAFTHWTARVLPAGSEVAVVEHAGHFLQLEQPDKIAELIVAFIGSPG