Gene Rv1145
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown. Thought to be involved in fatty acid transport. |
| Product | Probable conserved transmembrane transport protein MmpL13a |
| Comments | Rv1145, (MTCI65.12), len: 303 aa. Probable mmpL13a, conserved transmembrane transport protein (see citation below), member of RND superfamily, showing some similarity to putative Mycobacterial and Streptomyces membrane proteins e.g. MTCY987|g1781238 from Mycobacterium tuberculosis (962 aa), FASTA scores: opt: 213, E(): 1.9e-06, (28.0% identity in 296 aa overlap); etc. Strong similarity to U92075|MMU92075_5 hypothetical protein from Mycobacterium marinum (256 aa), FASTA scores: opt: 957, E(): 0, (57.6% identity in 257 aa overlap). Should continue as mmpL13B|Rv1146, but frameshift required. Sequence has been checked and is identical in M. tuberculosis strain CDC1551, and Mycobacterium bovis strain AF2122/97. Belongs to the MmpL family. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | DNA microarrays show higher level of expression in M. tuberculosis H37Rv during Mg2+ starvation (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene provides a growth advantage for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1272423 | 1273334 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1145|mmpL13a
MLQRIARLAIAAPRRIIGFAVFVFIAAAVFGVPVADSLSPGGFQDPRSESARAIEVLTDKFGQSGQKMLIVVTAAAGADSPPAREVGTDIVEVLRRSPLVYNVTSPWTVPPTAAADLLSTDGKSGLIVVNVKGGENDAQNHAQTLSDEVAHDRDGVTVRAGGSAMEYAQINRQNKDDLLVMELIAIPLSFLVLIWVFGGLLAAGLPMAQAVLAVVGSMAVLRLVTFATEVSTFALNLSTALGLALAIDYTLLIVSRYRDELAEGSDRDEALIRTMALRGARCCFRRSPWRCRCRRLRCSRCTF
Bibliography
- Tekaia F et al. [1999]. Analysis of the proteome of Mycobacterium tuberculosis in silico. Secondary Function
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
