Gene Rv1159
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Polyprenol-phosphate-mannose dependent mannosyltransferase involved in phosphatidylinositol mannoside synthesis |
| Product | Mannosyltransferase PimE |
| Comments | Rv1159, (MTCI65.26), len: 431 aa. PimE, mannosyltransferase (see Morita et al., 2006) Conserved transmembrane protein, similar to others in Mycobacterium tuberculosis e.g. Rv2181|MTCY21D4.13 (560 aa), FASTA scores: opt: 172; E(): 0.00035, (25.0% identity in 332 aa overlap). Belongs to the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1284992 | 1286287 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1159|pimE
MCRTLIDGPVRSAIAKVRQIDTTSSTPAAARRVTSPPARETRAAVLLLVLSVGARLAWTYLAPNGANFVDLHVYVSGAASLDHPGTLYGYVYADQTPDFPLPFTYPPFAAVVFYPLHLVPFGLIALLWQVVTMAALYGAVRISQRLMGGTAETGHFAAMLWTAIAIWIEPLRSTFDYGQINVLLMLAALWAVYTPRWWLSGLLVGVASGVKLTPAITAVYLVGVRRLHAAAFSVVVFLATVGVSLLVVGDEARYYFTDLLGDAGRVGPIATSFNQSWRGAISRILGHDAGFGPLVLAAIASTAVLAILAWRALDRSDRLGKLLVVELFGLLLSPISWTHHWVWLVPLMIWLIDGPARERPGARILGWGWLVLTIVGVPWLLSFAQPSIWQIGRPWYLAWAGLVYVVATLATLGWIAASERYVRIRPRRMAN
Bibliography
- Liu J et al. [2003]. Three monophyletic superfamilies account for the majority of the known glycosyltransferases. Homology
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Morita YS et al. [2006]. PimE is a polyprenol-phosphate-mannose-dependent mannosyltransferase that transfers the fifth mannose of phosphatidylinositol mannoside in mycobacteria. Function
- Bai G, Gazdik MA, Schaak DD and McDonough KA [2007]. The Mycobacterium bovis BCG cyclic AMP receptor-like protein is a functional DNA binding protein in vitro and in vivo, but its activity differs from that of its M. tuberculosis ortholog, Rv3676. Regulon
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
