Gene Rv1212c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism |
| Product | Putative glycosyl transferase GlgA |
| Comments | Rv1212c, (MTCI364.24c), len: 387 aa. Putative glgA, glycosyl transferase, highly similar to AJ243803|SCO243803_2 Putative glycosyl transferase from Streptomyces coelicolor (387 aa), FASTA scores: opt: 1344, E(): 0, (54.9% identity in 388 aa overlap). Also similar to MJ1607 probable hexosyltransferase from Methanococcus jannaschii (390 aa), FASTA scores: opt: 445, E(): 7.8e-23, (27.9% identity in 401 aa overlap). The region from aa 267-355 highly similar to Q49959 cosmid B1756 from Mycobacterium leprae (91 aa), FASTA scores, opt: 471, E(): 4.8e-25, (80.9% identity in 89 aa overlap). Similar to Mycobacterium tuberculosis hypothetical protein, Rv3032. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). M. tuberculosis H37Rv Rv1212c mutant produces less glucan; glycogen content is unaffected; growth in BALB/c mouse bone marrow macrophages is similar to wild-type; growth in BALB/c mice is reduced (See Sambou et al., 2008). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1354498 | 1355661 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1212c|glgA
MRVAMLTREYPPEVYGGAGVHVTELVAYLRRLCAVDVHCMGAPRPGAFAYRPDPRLGSANAALSTLSADLVMANAASAATVVHSHTWYTALAGHLAAILYDIPHVLTAHSLEPLRPWKKEQLGGGYQVSTWVEQTAVLAANAVIAVSSAMRNDMLRVYPSLDPNLVHVIRNGIDTETWYPAGPARTGSVLAELGVDPNRPMAVFVGRITRQKGVVHLVTAAHRFRSDVQLVLCAGAADTPEVADEVRVAVAELARNRTGVFWIQDRLTIGQLREILSAATVFVCPSVYEPLGIVNLEAMACATAVVASDVGGIPEVVADGITGSLVHYDADDATGYQARLAEAVNALVADPATAERYGHAGRQRCIQEFSWAYIAEQTLDIYRKVCA
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Sambou T et al. [2008]. Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: biosynthesis and impact on the persistence in mice. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
