Gene Rv1446c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | May be involved in the functional assembly of glucose 6-phosphate dehydrogenase |
| Product | Putative OXPP cycle protein OpcA |
| Comments | Rv1446c, (MTCY493.08), len: 303 aa. Putative opcA, OxPP cycle protein. Highly similar to S72774 B1496_F1_30 protein from Mycobacterium leprae (265 aa), FASTA scores: opt: 1056, E(): 0, (70.3% identity in 239 aa overlap). Also similar to OPCA_NOSS2|P48971 putative oxppcycle protein opca from Nostoc punctiforme (465 aa), fasta scores: opt: 177, E(): 7.3e-05, (23.4% identity in 321 aa overlap). Aids in G6PD activity. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Proteomics shows upregulation in isoniazid-resistant M. tuberculosis clinical isolates (See Jiang et al., 2006). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for survival in primary murine macrophages, by transposon site hybridization (TraSH) in H37Rv (See Rengarajan et al., 2005). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1624454 | 1625365 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1446c|opcA
MIVDLPDTTTTAVNKKLDELREKIGAVAMGRVLTLIIAPDSEAMLEESIEAANDASHEHPSRIIVTMRGDPYADRPRLDAQLRVGADAGAGEFVVLRLSGPLAGHADSVVIPFLLPDIPVVAWWPDIAPAVPAQDALGKLAIRRITDATNAIDPLSAIKSRLAGYGAGDTDLAWSRITYWRALLTSAVDQPRHEPIESALVSGLKTEPALDVLAGWLASRIEGPVRRAVGELKVELVRNSETIVLSRPQEGITATLTRTGKPDALVPLARRVTGECLAEDLRRLDPDEIYCAALEGIKKVQYR
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Rengarajan J et al. [2005]. Genome-wide requirements for Mycobacterium tuberculosis adaptation and survival in macrophages. Mutant
- Jiang X et al. [2006]. Comparison of the proteome of isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
