Gene Rv1512
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown, probably involved in nucleotide-sugar metabolism |
| Product | Probable nucleotide-sugar epimerase EpiA |
| Comments | Rv1512, (MTCY277.34), len: 322 aa. Probable epiA, nucleotide sugar epimerase, equivalent to AJ223832|MAS223832_4 from Mycobacterium avium silvaticum (339 aa), FASTA scores: opt: 1821, E(): 0, (84.6% identity in 318 aa overlap); and similar to WCAG_ECOLI|P32055 colanic acid biosynthesis protein wcaG (321 aa), FASTA scores: opt: 835, E(): 0, (53.5% identity in 316 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | The product of this CDS corresponds to spots 4_30, 4_31, 4_118, and 4_119 identified by proteomics at the Max Planck Institute for Infection Biology, Berlin, Germany (See Mattow et al., 2001). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1704093 | 1705061 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1512|epiA
MNAHTSVGPLDRAARVYIAGHRGLVGSALLRTFAGAGFTNLLVRSRAELDLTDRAATFDFVLESRPQVVIDAAARVGGILANDTYPADFLSENLQIQVNLLDAAVAARVPRLLFLGSSCIYPKLAPQPIPESALLTGPLEPTNDAYAIAKIAGILAVQAVRRQHGLPWISAMPTNLYGPGDNFSPSGSHLLPALIRRYDEAKASGAPNVTNWGTGTPRRELLHVDDLASACLYLLEHFDGPTHVNVGTGIDHTIGEIAEMVASAVGYSGETRWDPSKPDGTPRKLLDVSVLREAGWRPSIALRDGIEATVAWYREHAGTVRQ
Bibliography
- Mattow J, Jungblut PR, Schaible UE, Mollenkopf HJ, Lamer S, Zimny-Arndt U, Hagens K, Muller EC and Kaufmann SH [2001]. Identification of proteins from Mycobacterium tuberculosis missing in attenuated Mycobacterium bovis BCG strains. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
