Gene Rv1525
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly involved in cell wall arabinogalactan linker formation: Uses dTDP-L-rhamnose as substrate to insert the rhamnosyl residue into the cell wall. |
| Product | Possible rhamnosyl transferase WbbL2 |
| Comments | Rv1525, (MT1576, MTCY19G5.03c), len: 261 aa. Possible wbbL2, rhamnosyl transferase (see citation below), showing weak similarity to several rhamnosyl transferases. Similar to AF105060|AF105060_1 Riftia pachyptila endosymbiont (746 aa), FASTA scores: opt: 183, E(): 0.00013, (35.2% identity in 105 aa overlap). |
| Functional category | Conserved hypotheticals |
| Transcriptomics | DNA microarrays show lower level of expression in M. tuberculosis H37Rv than in phoP|Rv0757 mutant (See Walters et al., 2006). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1720017 | 1720802 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1525|wbbL2
LYAPLVSLMITVPVFGQHEYTHALVADLEREGADYLIVDNRGDYPRIGTERVSTPGENLGWAGGSELGFRLAFAEGYSHAMTLNNDTRVSKGFVAALLDSRLPADAGMVGPMFDVGFPFAVADEKPDAESYVPRARYRKVPAVEGTALVMSRDCWDAVGGMDLSTFGRYGWGLDLDLALRARKSGYGLYTTEMAYINHFGRKTANTHFGGHRYHWGASAAMIRGLRRTHGWPAAMGILREMGMAHHRKWHKSFPLTCPASC
Bibliography
- Belanger AE and Inamine JM [2000]. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=&dopt=Abstract Review
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Walters SB et al. [2006]. The Mycobacterium tuberculosis PhoPR two-component system regulates genes essential for virulence and complex lipid biosynthesis. Transcriptome
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
