Gene Rv1529
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown, but involvement in lipid degradation. |
| Product | Probable fatty-acid-AMP ligase FadD24 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) |
| Comments | Rv1529, (MTV045.03), len: 584 aa. Probable fadD24, fatty-acid-AMP synthetase, highly similar to many e.g. MBU75685_1|AAB52538.1|U75685 acyl-CoA synthase from Mycobacterium bovis (582 aa), FASTA score: (65.6% identity in 582 aa overlap); and many other fatty-acid-CoA synthetases from Mycobacteria e.g. fadD25|MTCY19G5_7 from Mycobacterium tuberculosis (583 aa), FASTA score: (68.7% identity in 584 aa overlap); fadD28|MTCY24G1_8 from Mycobacterium tuberculosis (580 aa), FASTA score: (66.0% identity in 582 aa overlap); NP_301232.1|NC_002677|U00010_6 from Mycobacterium leprae (372 aa), FASTA score: (57.6% identity in 342 aa overlap); FADD23|Rv3826|MTCY409.04c from Mycobacterium tuberculosis (584 aa), FASTA score: (63.2% identity in 584 aa overlap); etc. |
| Functional category | Lipid metabolism |
| Proteomics | Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1729502 | 1731256 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1529|fadD24
VVASSIPTALRERASVHPNGAAITYIDYEQDWAGVAETLTWSQLYRRMLNVAEPLRHVGATGDRAVILAPQGIEYVVGFLGALQAGRIAVPLPVPHAGAHDERTISVLSDTSPAVILTTSGAVDDVRECAQPQPGQSAPSIVELDLLDLDSRQRSRSPGARPTGRDTPETAYLQYTSGSTRTPAGVMVSNKNVFANFEQIVADFFAPEGGVVPPDLTVVSWLPLYHDMGLLLGAIMPILAGVPTVLTSPVGFLQRPARWIQLLARNGRTISAGPNFAFELAVRKTSDDDMDGLDLAGVHTILNGSERVHPATLKRFAERFGRFNFAAAALRPAYGMAEATVYIATRNVNEPPEIVDFESEKLPAGQAIRCPSGSGTPLVSYGVPRSQLVRIVDPDTCIECPQGSVGEIWVQGGNVASGYWHKPEESKRTFGARIVTPSAGTPEAPWLRTGDSGFVSGGELFIIGRIKDLLIVYGRNHAPDDIEATIQEITSGRCAAIAVPDHGTEKLVAIIELKKRGDSDEDVADRLRIVKRDVAAAIFDSHGLSVADLVLVSPGSIPITTSGKIRRAQCVQLYRRREFTRLDA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Trivedi OA et al. [2004]. Enzymic activation and transfer of fatty acids as acyl-adenylates in mycobacteria. Function Product
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
