Gene Rv1565c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Conserved hypothetical membrane protein |
| Comments | Rv1565c, (MTCY336.38), len: 729 aa. Conserved hypothetical membrane protein, some similarity to O05402 hypothetical 72.2 kDa protein from Bacillus subtilis (634 aa), FASTA results: opt: 384, E(): 4.8e-17, (29.1% identity in 378 aa overlap); and to Y392_HAEIN|P43993 hypothetical protein hi0392 from H. influenzae (245 aa), FASTA results: opt: 265, E(): 5.5e-10, (28.3% identity in 247 aa overlap). C-terminal half equivalent to AL049478|MLCL458_19 (274 aa) (78.5% identity in 274 aa overlap). Also similar to Mycobacterium tuberculosis hypothetical proteins Rv0111, Rv0228, Rv1254, Rv0517. N-terminal half hydrophobic. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1771640 | 1773829 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1565c|Rv1565c
MLTLSPPRPPALTPEPALPPVTMGTRTTGFYRHDLDGLRGVAIALVAVFHVWFGRVSGGVDVFLALSGFFFGGKILRAALNPDLSLSPIAEVIRLIRRLLPALVVVLAGCALLTIAIQPQTRWEAFANQSLASLGYYQNWELASTVSNYLRAGEAVSPLQHIWSMSVQGQFYLAFLLLVAGCAYLLRRLFRGPRAPYLRTMFVVLLSTLTLASFIYAIVAHHAYQATAYYNTFARAWELLAGALVGAVVPHVRWPMWLRTAVATAALAAILSCGALIDGVKEFPGPWALVPVGATMLMILAGANRQGHPGTRDRLPLPNRLLATAPLVALGAMAYSWYLWHWPLLIFWLSYTGHRHANFVEGAAVLLVSGLLAYLTTRLVEDPLRYRAPAGVRSPAAVPPIPWRLRLRRPTIVLGSVVALLGVALTATSFTWREHVIVQRAAGKELSGLSSRDYPGARALIDHVRVPKLRMRPTVLEVRHDLPTSTKDGCISDFVNPAIINCTYGDVDAPRTIALAGGSHAEHWLTALDLLGRMHHFKVVTYLKMGCPLSTEEVPLIMGNNAPYPQCHQWVQAAMAKLVADHPDYVFTTSTRPWNIKPGDVMPATYVGIWQTFADNNIPVLAMRDTPWLVKDGQPFIPADCLAKGGNPQSCGIARSKVLVDRNPTLDFVARFPLLKPLDMSDAICRTDTCRAVEGNVLVYRDSHHLTPTYMRTMTSELGRQIAANTDWW
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
