Gene Rv1627c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in lipid metabolism. |
| Product | Probable nonspecific lipid-transfer protein |
| Comments | Rv1627c, (MTCY01B2.19c), len: 402 aa. Probable nonspecific lipid-transfer protein, similar to many lipid carrier proteins e.g. Q51797 acetyl CoA synthase from Pyrococcus furiosus (388 aa), FASTA scores: opt: 400, E(): 3.2e-18, (34.4% identity in 407 aa overlap); etc. Also some similarity to Mycobacterium tuberculosis proteins Rv3523, Rv3540c, Rv0244, Rv2790c, Rv1323, etc. |
| Functional category | Lipid metabolism |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Detected by 2-DE and MS in M. tuberculosis H37Rv purified from phagosomes of infected murine bone marrow macrophages but not in H37Rv broth-cultures (See Mattow et al., 2006). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv on cholesterol, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1828865 | 1830073 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1627c|Rv1627c
MRMSAPEPVYILGAGMHPWGKWGNDFTEYGVVAARAALRDAGVDWRHVQLVAGADTIRNGYPGFVAGATFAQKLGWTGVPVSSSYAACASGSQALQSARAQILAGFCDVALVIGADTTPKGFFAPVGGERKGDPDWQRFHLIGATNTVYFALLARRRMDLYGATVEDFAQVKVKNSRHGLDNPNARYRKENSIDDVLASPVVSDPLRLLDICATSDGAAALIVASKSFTEKHLGSVAGVPSVRAISTVTPKYPQHLPELPDIATDSTAAVPAPERVFKDQILDAAYAEAGIGPEDLSLAEVYDLSTALELDWYEHLGLCPKGEAEALLRSGATTLGGRVPVNPSGGLACFGEAIPAQAIAQVCELTWQLRGQATGRQVADAKVGVTANQGLFGHGSSVIVAR
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mattow J, Siejak F, Hagens K, Becher D, Albrecht D, Krah A, Schmidt F, Jungblut PR, Kaufmann SH and Schaible UE [2006]. Proteins unique to intraphagosomally grown Mycobacterium tuberculosis. Proteomics
- Kendall SL, Withers M, Soffair CN, Moreland NJ, Gurcha S, Sidders B, Frita R, Ten Bokum A, Besra GS, Lott JS and Stoker NG [2007]. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Regulation
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
