Gene Rv1753c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | PPE family protein PPE24 |
| Comments | Rv1753c, (MTCY28.16c), len: 1053 aa. PPE24, Member of the Mycobacterium tuberculosis PPE family of Gly-, Asn-rich proteins, similar to many e.g. YF48_MYCTU|Q10778 hypothetical protein cy48.17 (678 aa), FASTA scores: opt: 1360, E(): 0, (48.9% identity in 550 aa overlap). Note that the Gly-, Asn-rich sequence is interrupted by six near-perfect 26 aa repeats, a unique region, and another, more degenerate region of five 25 aa repeats before resuming at the C-terminus. The end of the first Gly-, Asn- rich region and the start of the first set of repeats shows some similarity to Q50577|AT10S from Mycobacterium tuberculosis (170 aa) (40.2% identity in 189 aa overlap). |
| Functional category | Pe/ppe |
| Proteomics | Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 1981614 | 1984775 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1753c|PPE24
MNFSVLPPEINSALIFAGAGPEPMAAAATAWDGLAMELASAAASFGSVTSGLVGGAWQGASSSAMAAAAAPYAAWLAAAAVQAEQTAAQAAAMIAEFEAVKTAVVQPMLVAANRADLVSLVMSNLFGQNAPAIAAIEATYEQMWAADVSAMSAYHAGASAIASALSPFSKPLQNLAGLPAWLASGAPAAAMTAAAGIPALAGGPTAINLGIANVGGGNVGNANNGLANIGNANLGNYNFGSGNFGNSNIGSASLGNNNIGFGNLGSNNVGVGNLGNLNTGFANTGLGNFGFGNTGNNNIGIGLTGNNQIGIGGLNSGTGNFGLFNSGSGNVGFFNSGNGNFGIGNSGNFNTGGWNSGHGNTGFFNAGSFNTGMLDVGNANTGSLNTGSYNMGDFNPGSSNTGTFNTGNANTGFLNAGNINTGVFNIGHMNNGLFNTGDMNNGVFYRGVGQGSLQFSITTPDLTLPPLQIPGISVPAFSLPAITLPSLNIPAATTPANITVGAFSLPGLTLPSLNIPAATTPANITVGAFSLPGLTLPSLNIPAATTPANITVGAFSLPGLTLPSLNIPAATTPANITVGAFSLPGLTLPSLNIPAATTPANITVGAFSLPGLTLPSLNIPAATTPANITVSGFQLPPLSIPSVAIPPVTVPPITVGAFNLPPLQIPEVTIPQLTIPAGITIGGFSLPAIHTQPITVGQIGVGQFGLPSIGWDVFLSTPRITVPAFGIPFTLQFQTNVPALQPPGGGLSTFTNGALIFGEFDLPQLVVHPYTLTGPIVIGSFFLPAFNIPGIDVPAINVDGFTLPQITTPAITTPEFAIPPIGVGGFTLPQITTQEIITPELTINSIGVGGFTLPQITTPPITTPPLTIDPINLTGFTLPQITTPPITTPPLTIDPINLTGFTLPQITTPPITTPPLTIEPIGVGGFTTPPLTVPGIHLPSTTIGAFAIPGGPGYFNSSTAPSSGFFNSGAGGNSGFGNNGSGLSGWFNTNPAGLLGGSGYQNFGGLSSGFSNLGSGVSGFANRGILPFSVASVVSGFANIGTNLAGFFQGTTS
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
