Gene Rv1992c (cmtA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Metal cation-transporting ATPase; possibly catalyzes the transport of an undetermined metal cation with the hydrolysis of ATP [catalytic activity: ATP + H(2)O + undetermined metal cation(in) = ADP + phosphate + undetermined metal cation(out)]. |
| Product | Probable metal cation transporter P-type ATPase G CtpG |
| Comments | Rv1992c, (MTCY39.27), len: 771 aa. Probable ctpG, metal cation-transporting P-type ATPase G (transmembrane protein), similar to others, especially cadmium-transporting ATPases, e.g. NP_244904.1|NC_002570 cadmium-transporting ATPase from Bacillus halodurans (707 aa); P30336|CADA_BACFI probable cadmium-transporting ATPase from Bacillus firmus (723 aa); BAB47609.1|AB037671 cadmium resistance protein B from Staphylococcus aureus (804 aa); 3121832|Q60048|CADA_LISMO probable cadmium-transporting ATPase from Listeria monocytogenes (707 aa); etc. Also similar to others from Mycobacterium tuberculosis e.g. Rv0969|MTCY10D7.05c|ctpV putative cation transporter P-type ATPase V (770 aa); Rv1469; Rv0092; etc. Contains PS00435 Peroxidases proximal heme-ligand signature and PS00154 E1-E2 ATPases phosphorylation site. Belongs to the cation transport ATPases family (E1-E2 ATPases), subfamily IB. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS; predicted transmembrane protein (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS; predicted integral membrane protein (See Xiong et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Operon | Rv1994c, Rv1993c, and Rv1992c are co-transcribed in BCG (See Cavet et al., 2003) and M. tuberculosis H37Rv, by RT-PCR (See Chauhan et al., 2009). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Found to be deleted (partially or completely) in one or more clinical isolates (See Tsolaki et al., 2004). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2234991 | 2237306 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv1992c|ctpG
VTTVVDAEVQLTVVSDAAGRMRVQATGFQFDAGRAVAIEDTVGKVAGVQAVHAYPRTASIVIWYSRAICDTAAILSAIIDAETVPAAAVPAYASRSASNRKAGVVQKIIDWSTRTLSGVRRDVAAQPSGETSDACCDGEDNEDREPEQLWQVAKLRRAAFSGVLLTASLVAAWAYPLWPVVLGLKALALAVGASTFVPSSLKRLAEGRVGVGTLMTIAALGAVALGELGEAATLAFLFSISEGLEEYATARTRRGLRALLSLVPDQATVLREGTETIVASTELHVGDQMIVKPGERLATDGIIRAGRTALDVSAITGESVPVEVGPGDEVFAGSINGLGVLQVGVTATAANNSLARIVHIVEAEQVRKGASQRLADCIARPLVPSIMIAAALIAGTGSVLGNPLVWIERALVVLVAAAPCALAIAVPVTVVASIGAASRLGVLIKGGAALETLGTIRAVALDKTGTLTANRPVVIDVATTNGATREEVLAVAAALEARSEHPLAVAVLAATQATTAASDVQAVPGAGLIGRLDGRVVRLGRPGWLDAAELADHVACMQQAGATAVLVERDQQLLGAIAVRDELRPEAAEVVAGLRTGGYQVTMLTGDNHATAAALAAQAGIEQVHAELRPEDKAHLVAQLRARQPTAMVGDGVNDAPALAAADLGIAMGAMGTDVAIETADVALMGQDLRHLPQALDHARRSRQIMVQNVGLSLSIITVLMPLALFGILGLAAVVLVHEFTEVIVIANGVRAGRIKPLAGPPKTPDRTIPG
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Cavet JS et al. [2003]. A cadmium-lead-sensing ArsR-SmtB repressor with novel sensory sites. Complementary metal discrimination by NmtR AND CmtR in a common cytosol. Operon
- Tsolaki AG, Hirsh AE, DeRiemer K, Enciso JA, Wong MZ, Hannan M, Goguet de la Salmoniere YO, Aman K, Kato-Maeda M and Small PM [2004]. Functional and evolutionary genomics of Mycobacterium tuberculosis: insights from genomic deletions in 100 strains. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Chauhan S et al. [2009]. CmtR, a cadmium-sensing ArsR-SmtB repressor, cooperatively interacts with multiple operator sites to autorepress its transcription in Mycobacterium tuberculosis. Operon
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
