Gene Rv2045c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Converts unknown esters to corresponding free acid and alcohol |
| Product | Carboxylesterase LipT |
| Comments | Rv2045c, (MTV018.32c), len: 511 aa. LipT, carboxylesterase, similar to many. Contains PS00941 Carboxylesterases type-B signature 2. Contains PS00122 Carboxylesterases type-B serine active site. |
| Functional category | Intermediary metabolism and respiration |
| Transcriptomics | mRNA identified by microarray analysis and up-regulated after 96h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2289685 | 2291220 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2045c|lipT
VALESATVGSMHERTVRARTATGIVEGFTRDGVHRWRSIPYARAPVGSLRFRAPQPAQPWPGVRHCHTFANCAPQQRRYTVMGIGRYQTRSEDCLTLNVVTPEEPATQPLPVMVFIHGGGYILGSSATPIYDGAALARRGCVYVSVNYRLGALGCLDLSSLSTPQITLDSNVYLRDLVLALRWVHDNIAEFGGDPGNVTIFGESAGAHITATLLAVPAAKGLFARAISESPAAGMVRSREVAAEFAARFANLIGARTQDAANALMQASPAQLVEAQHHLIRQGMRKRLGAFPIGPVFGDDYLPMDPVEAMRSGRVHAVPLIVGTNAEEGRLFTRFLGMLPTNEPMVEELLSGMKPADRERITAAYPNYPAPSACIQLGGDFAFSSAAWQIAEAHGANAPTYLYRYDYAPRTLRWSGFGATHATELFAVFDIYRTRFGALLTAAADRRAALRVSNEVQRRWRCFSQIGVPGDDWPAYTQDDRAVLVFDRRCRIEFDPHQHRRIAWDGFSLAN
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Deb C, Daniel J, Sirakova TD, Abomoelak B, Dubey VS and Kolattukudy PE [2006]. A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis. Product Transcriptome
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
