Gene Rv2174
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Mannosyltransferase, likely involved in the biosynthesis of lipomannan (LM) |
| Product | Alpha(1->6)mannosyltransferase. Possible conserved integral membrane protein. |
| Comments | Rv2174, (MTV021.07), len: 516 aa. MptA (mannopyranosyltransferase A) (See Mishra et al., 2007). Possible conserved integral membrane protein, similar to some hypothetical mycobacterial proteins e.g. Mycobacterium leprae ML0899 probable integral-membrane protein (505 aa) and MLCL536_26 (593 aa). FASTA scores: ML0899 opt: 2715; 78.884% identity in 502 aa overlap and gp|Z99125|MLCL536_26 Mycobacterium leprae cosmid L536. (593 aa) opt: 552, E(): 7.1e-30; 31.6% identity in 513 aa overlap. Also similar to Rv1459c. Predicted to be in the GT-C superfamily of glycosyltransferases (See Liu and Mushegian, 2003). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2435909 | 2437459 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2174|mptA
MTTPSHAPAVDLATAKDAVVQHLSRLFEFTTGPQGGPARLGFAGAVLITAGGLGAGSVRQHDPLLESIHMSWLRFGHGLVLSSILLWTGVGVMLLAWLGLGRRVLAGEATEFTMRATTVIWLAPLLLSVPVFSRDTYSYLAQGALLRDGLDPYAVGPVGNPNALLDDVSPIWTITTAPYGPAFILVAKFVTVIVGNNVVAGTMLLRLCMLPGLALLVWATPRLASHLGTHGPTALWICVLNPLVLIHLMGGVHNEMLMVGLMTAGIALTVQGRNVAGIILITVAIAVKATAGIALPFLVWVWLRHLRERRGYRPVQAFLAAAAISLLIFVAVFAVLSAVAGVGLGWLTALAGSVKIINWLTVPTGAANVIHALGRGLFTVDFYTLLRITRLIGIVIIAVSLPLLWWRFRRDDRAALTGVAWSMLIVVLFVPAALPWYYSWPLAVAAPLAQARRAIAAIAGLSTWVMVIFKPDGSHGMYSWLHFWIATACALTAWYVLYRSPDRRGVQAATPVVNTP
Bibliography
- Liu J et al. [2003]. Three monophyletic superfamilies account for the majority of the known glycosyltransferases. Homology
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mishra AK et al. [2007]. Identification of an alpha(1-->6) mannopyranosyltransferase (MptA), involved in Corynebacterium glutamicum lipomanann biosynthesis, and identification of its orthologue in Mycobacterium tuberculosis. Product Function
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
