Gene Rv2226
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Conserved protein |
| Comments | Rv2226, (MTCY427.07), len: 513 aa. Conserved protein, similar to hypothetical secreted protein (510 aa) from Streptomyces coelicolor A3(2) emb|CAB59601.1| (AL132662) hypothetical secreted protein [Streptomyces coelicolor. Smith-Waterman scores Expect = 5e-44 Identities = 166/506 (32%) |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2498832 | 2500373 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2226|Rv2226
MPVEAPRPARHLEVERKFDVIESTVSPSFEGIAAVVRVEQSPTQQLDAVYFDTPSHDLARNQITLRRRTGGADAGWHLKLPAGPDKRTEMRAPLSASGDAVPAELLDVVLAIVRDQPVQPVARISTHRESQILYGAGGDALAEFCNDDVTAWSAGAFHAAGAADNGPAEQQWREWELELVTTDGTADTKLLDRLANRLLDAGAAPAGHGSKLARVLGATSPGELPNGPQPPADPVHRAVSEQVEQLLLWDRAVRADAYDAVHQMRVTTRKIRSLLTDSQESFGLKESAWVIDELRELADVLGVARDAEVLGDRYQRELDALAPELVRGRVRERLVDGARRRYQTGLRRSLIALRSQRYFRLLDALDALVSERAHATSGEESAPVTIDAAYRRVRKAAKAAKTAGDQAGDHHRDEALHLIRKRAKRLRYTAAATGADNVSQEAKVIQTLLGDHQDSVVSREHLIQQAIAANTAGEDTFTYGLLYQQEADLAERCREQLEAALRKLDKAVRKARD
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
