Gene Rv2291
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown |
| Product | Probable thiosulfate sulfurtransferase SseB |
| Comments | Rv2291, (MTCY339.19c), len: 284 aa. Probable sseB, thiosulfate sulfurtransferase. Very similar to thiosulfate sulfurtransferas/rhodanese from Streptomyces coelicolor AL00920 4|SC9B10_21 (283 aa) opt: 765, E(): 0; Smith-Waterman score: 765; 46.9% identity in 286 aa overlap, similar to THTR_ECOLI P31142 putative thiosulfate sulfurtransferase (280 aa), FASTA scores, opt: 478, E(): 1e-23, (35.1% identity in 265 aa overlap) |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2563174 | 2564028 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2291|sseB
VQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELSDHTIAGRGRHPLPSGASLQATVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVRILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQRLTLTAQQAGAGGVTLLDARVPERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVYCGSGVSAAVIVAALAVIGQDAELFPGSWSEWSSDPTRPVGRGTA
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
