Gene Rv2318
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Thought to be involved in active transport of sugar across the membrane (import). |
| Product | Probable periplasmic sugar-binding lipoprotein UspC |
| Comments | Rv2318, (MTCY3G12.16c), len: 440 aa. Probable uspC, sugar-binding lipoprotein component of sugar transport system (see citation below), most similar to Q9CBN6|USPC|ML1770 sugar transport periplasmic binding protein from Mycobacterium leprae (446 aa), FASTA scores: opt: 2294, E(): 8.1e-135, (74.7% identity in 446 aa overlap). Also similar to other substrate-binding proteins e.g. Q9RK89|SCF1.15 putative substrate binding protein (extracellular) (binding-protein-dependent transport) (fragment) from Streptomyces coelicolor (221 aa), FASTA scores: opt: 377, E(): 3e-16, (32.25% identity in 217 aa overlap); Q9K6N8|BH3690 sugar transport system (sugar-binding protein) from Bacillus halodurans (420 aa), FASTA scores: opt: 227, E(): 1e-06, (25.00% identity in 452 aa overlap); etc. Also similar to O53485|Rv2041c|MTV018.28C lipoprotein component of sugar transport system from Mycobacterium tuberculosis (439 aa), FASTA scores: opt: 246, E(): 7e-08, (26.75% identity in 325 aa overlap). Contains a hydrophobic stretch (possible signal peptide) at N-terminal end. |
| Functional category | Cell wall and cell processes |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2590518 | 2591840 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2318|uspC
VTRPRQSTLVATALVLVAILLGVTAVLLGLSAEPRGGKIVVTVRLWDEPIAAAYRQSFAAFTRSHPDIEVRTNLVAYSTYFETLRTDVAGGSADDIFWLSNAYFAAYADSGRLMKIQTDAADWEPAVVDQFTRSGVLWGVPQLTDAGIAVFYNADLLAAAGVDPTQVDNLRWSRGDDDTLRPMLARLTVDADGRTANTPGFDARRVRQWGYNAANDPQAIYLNYIGSAGGVFQRDGKFAFDNPGAIEAFRYLVGLINDDHVAPPASDTNDNGDFSRNQFLAGKMALFQSGTYSLAPVARDALFHWGVAMLPAGPAGRVSVTNGIAAAGNSASKHPDAVRQVLAWMGSTEGNSYLGRHGAAIPAVLSAQPVYFDYWSARGVDVTPFFAVLNGPRIAAPGGAGFAAGQQALEPYFDEMFLGRGDVTTTLRQAQAAANAATQR
Bibliography
- Braibant M et al. [2000]. The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. Review Secondary
- Titgemeyer F et al. [2007]. A genomic view of sugar transport in Mycobacterium smegmatis and Mycobacterium tuberculosis. Homology
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
