Gene Rv2339
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Unknown. Thought to be involved in fatty acid transport. |
| Product | Probable conserved transmembrane transport protein MmpL9 |
| Comments | Rv2339, (MTCY98.08), len: 962 aa. Probable mmpL9, conserved transmembrane transport protein (see citation below), with strong similarity to other Mycobacterial proteins e.g. P54881|YV34_MYCLE|MML4_MYCLE hypothetical 105.2 kDa protein from Mycobacterium leprae (959 aa), FASTA scores: opt: 3799, E(): 0, (59.3% identity in 937 aa overlap); G699237|U1740AB from Mycobacterium leprae; and MTCY20G9.34; MTCY48.08c; MTCY19G5.06 from Mycobacterium tuberculosis. Belongs to the MmpL family. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2614693 | 2617581 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2339|mmpL9
MVPGEVHMSDTPSGPHPIIPRTIRLAAIPILLCWLGFTVFVSVAVPPLEAIGETRAVAVAPDDAQSMRAMRRAGKVFNEFDSNSIAMVVLESDQPLGEKAHRYYDHLVDTLVLDQSHIQHIQDFWRDPLTAAGAVSADGKAAYVQLYLAGNMGEALANESVEAVRKIVANSTPPEGIRTYVTGPAALFADQIAAGDRSMKLITGLTFAVITVLLLLVYRSIATTLLILPMVFIGLGATRGTIAFLGYHGMVGLSTFVVNILTALAIAAGTDYAIFLVGRYQEARHIGQNREASFYTMYRGTANVILGSGLTIAGATYCLSFARLTLFHTMGPPLAIGMLVSVAAALTLAPAIIAIAGRFGLLDPKRRLKTRGWRRVGTAVVRWPGPILATSVALALVGLLALPGYRPGYNDRYYLRAGTPVNRGYAAADRHFGPARMNPEMLLVESDQDMRNPAGMLVIDKIAKEVLHVSGVERVQAITRPQGVPLEHASIPFQISMMGATQTMSLPYMRERMADMLTMSDEMLVAINSMEQMLDLVQQLNDVTHEMAATTREIKATTSELRDHLADIDDFVRPLRSYFYWEHHCFDIPLCSATRSLFDTLDGVDTLTDQLRALTDDMNKMEALTPQFLALLPPMITTMKTMRTMMLTMRSTISGVQDQMADMQDHATAMGQAFDTAKSGDSFYLPPEAFDNAEFQQGMKLFLSPNGKAVRFVISHESDPASTEGIDRIEAIRAATKDAIKATPLQGAKIYIGGTAATYQDIRDGTKYDILIVGIAAVCLVFIVMLMITQSLIASLVIVGTVLLSLGTAFGLSVLIWQHFVGLQVHWTIVAMSVIVLLAVGSDYNLLLVSRFKEEVGAGLKTGIIRAMAGTGAVVTSAGLVFAFTMASMAVSELRVIGQVGTTIGLGLLFDTLVVRSFMTPSIAALLGRWFWWPNMIHSRPTVPEAHTRQGARRIQPHLHRG
Bibliography
- Tekaia F et al. [1999]. Analysis of the proteome of Mycobacterium tuberculosis in silico. Secondary Function
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
