Gene Rv2358
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Probable transcriptional regulatory protein SmtB (probably ArsR-family) |
| Comments | Rv2358, (MTCY27.22c), len: 135 aa. Probable smtB, transcriptional regulator, arsR family, equivalent to Q9CCG5|ML0825 putative ArsR-family transcriptional regulator from Mycobacterium leprae (140 aa), FASTA scores: opt: 647, E(): 2e-34, (72.9% identity in 140 aa overlap). Also similar to others e.g. BAB48273|MLR0745 Transcriptional regulator from Rhizobium loti (Mesorhizobium loti) (104 aa), FASTA scores: opt: 185, E(): 3.4e-05, (43.25% identity in 74 aa overlap) (has its N-terminus shorter); P15905|ARR1_ECOLI arsenical resistance operon repressor from Escherichia coli (117 aa), FASTA scores: opt: 164, E(): 8.1e-05, (39.1% identity in 69 aa overlap); etc. Also similar to O53838|Rv0827|MTV043.19c putative transcriptional regulator from Mycobacterium tuberculosis (130 aa), FASTA scores: opt: 201, E(): 4e-06, (35.7% identity in 98 aa overlap); and O69711|Rv3744|MTV025.092 putative regulatory protein from Mycobacterium tuberculosis (120 aa), FASTA scores: opt: 209, E(): 1.2e-06, (35.5 % identity in 93 aa overlap). Contains possible helix-turn-helix motif at aa 72-93 (Score 1103, +2.94 SD). Belongs to the ArsR family of transciptional regulators. Shown to bind palindromic DNA sequence upstream of Rv2358; inhibited by Zn2+ (See Canneva et al., 2005). |
| Functional category | Regulatory proteins |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2641246 | 2641653 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2358|smtB
MVTSPSTPTAAHEDVGADEVGGHQHPADRFAECPTFPAPPPREILDAAGELLRALAAPVRIAIVLQLRESQRCVHELVDALHVPQPLVSQHLKILKAAGVVTGERSGREVLYRLADHHLAHIVLDAVAHAGEDAI
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Milano A et al. [2004]. The Mycobacterium tuberculosis Rv2358-furB operon is induced by zinc. Regulation
- Canneva F et al. [2005]. Rv2358 and FurB: two transcriptional regulators from Mycobacterium tuberculosis which respond to zinc. Regulation
- Campbell DR et al. [2007]. Mycobacterial cells have dual nickel-cobalt sensors: sequence relationships and metal sites of metal-responsive repressors are not congruent. Biochemistry
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
