Gene Rv2363
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Generates monocarboxylate from monocarboxylic acid amide [catalytic activity: a monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)]. |
| Product | Probable amidase AmiA2 (aminohydrolase) |
| Comments | Rv2363, (MTCY27.17c), len: 484 aa. Probable amiA2, amidase, highly similar or similar to others e.g. O28325|YJ54_ARCFU|AF1954 putative amidase from Archaeoglobus fulgidus (453 aa), FASTA scores: opt: 777, E(): 1.1e-38, (35.0% identity in 474 aa overlap); Q55424|AMID_SYNY3|SLL0828 putative amidase from Synechocystis sp. strain PCC 6803 (506 aa), FASTA scores: opt: 770, E(): 3e-38, (36.4% identity in 456 aa overlap); Q53116|AMDA enantiomerase-selective amidase from Rhodococcus sp. (462 aa), FASTA scores: opt: 701, E(): 3.5e-34, (32.7% identity in 468 aa overlap); etc. Also highly similar to others from Mycobacterium tuberculosis e.g. AMI2_MYCTU|AMIB2|Q11056|Rv1263|MT1301|MTCY50.19c|cy50.19c amidase (462 aa), FASTA scores: opt: 1141, E(): 2.9e-60, (45.4% identity in 454 aa overlap); etc. Contains PS00571 Amidases signature, and PS00017 ATP/GTP-binding site motif A (P-loop). Belongs to the amidase family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 days but not 90 days (See Kruh et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2644320 | 2645774 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2363|amiA2
VVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDVSQPTLNAFRVVLTESALADAAAADKRRAAGDTAPLLGIPIAVKDDVDVAGVPTAFGTQGYVAPATDDCEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSRRHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVLDAASGNVEGDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFRAKLHPEILAATQRVGDQLELLGHTVVKGNPDYGLRLSWNFLARSTAGLWEWAERLGDEVTLDRRTVSNLRMGHVLSQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPMARAFDRLGSFGTDRAIIAACPSTWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQVWWTS
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
