Gene Rv2410c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv2410c, (MTCY253.10), len: 325 aa. Conserved protein, equivalent to Q49770|CAC30114|ML0606 conserved hypothetical protein from Mycobacterium leprae (325 aa), FASTA scores: opt: 1928, E(): 3.5e-117, (90.75% identity in 325 aa overlap). Also some similarity with other hypothetical proteins e.g. Q9RST2|DR2041 conserved hypothetical protein from Deinococcus radiodurans (316 aa), FASTA scores: opt: 329, E(): 5.3e-14, (32.4% identity in 318 aa overlap); C-terminus of Q9HUN7|PA4927 hypothetical protein from Pseudomonas aeruginosa (830 aa), FASTA scores: opt: 297, E(): 1.5e-11, (27.6% identity in 315 aa overlap); etc. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2707333 | 2708310 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2410c|Rv2410c
MLARNAEALYWIGRYVERADDTARILDVAVHQLLEDSSVDPDQASRLLLRVLGIEPPDHELDVWSLTDLVAFSTNSQGGSSIVDAISAARENAKSAREVTSSETWECLNTTYNALPERERAAKRLGPHEFLSFIEGRAAMFAGLADSTLLRDDGYRFMLLGRAIERVDMTVRLLLSRVGDSASSPAWVTLLRSAGAHDTYLRTYRGVLDAGRVVEFMMLDRLFPRSVFHSLKLAEHNLAELMHNPHSRIGATTEAQRLLGQARSELEFVQPGVLLETLESRLAGLQTTCRDVGDALALQYFHAAPWVAWSDAGQRGQLVGSQEES
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
