Gene Rv2416c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Acetylation, substrate unknown. Involved in intracellular survival. Possibly associated with the cell surface and secreted. Modulates cytokine secretion by host immune cells. |
| Product | Enhanced intracellular survival protein Eis, GCN5-related N-acetyltransferase |
| Comments | Rv2416c, (MTCY253.04), len: 402 aa. Eis, enhanced intracellular survival gene (see citations below). Conserved hypothetical protein, contains GNAT (Gcn5-related N-acetyltransferase) domain in N-terminal part, similar to Q9F309|SCC80.10 hypothetical 44.7 KDA protein from Streptomyces coelicolor (413 aa), FASTA scores: opt: 382, E(): 1e-16, (31.45% identity in 407 aa overlap); Q9K4F4|SCD66.23 conserved hypothetical protein from Streptomyces coelicolor (418 aa), FASTA scores: opt: 238, E(): 1.3e-07, (36.5% identity in 364 aa overlap): and Q54238|G1139577|ORF5 hypothetical protein from Streptomyces griseus (416 aa), FASTA scores: opt: 237, E(): 1.5e-07, (34.0 identity in 423 aa overlap). Start changed since first submission (- 6 aa) (see Dahl et al., 2001; Wei et al., 2000; Vetting et al. 2005). |
| Functional category | Virulence, detoxification, adaptation |
| Proteomics | Identified by proteomics at the Statens Serum Institute (Denmark) (see Rosenkrands et al., 2000). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene for in vitro growth of H37Rv on cholesterol, by sequencing of Himar1-based transposon mutagenesis (See Griffin et al., 2011). Survival of M. tuberculosis H37Rv eis|Rv2416c mutant in U-937 macrophages and in C57BL/6 mice is not significantly different from wild-type (See Samuel et al., 2007). Mutations in the promoter region of eis|Rv2416c in M. tuberculosis H37Rv result in increased expression of eis|Rv2416c and kanamycin resistance (See Zaunbrecher et al., 2009). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2714124 | 2715332 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2416c|eis
VTVTLCSPTEDDWPGMFLLAAASFTDFIGPESATAWRTLVPTDGAVVVRDGAGPGSEVVGMALYMDLRLTVPGEVVLPTAGLSFVAVAPTHRRRGLLRAMCAELHRRIADSGYPVAALHASEGGIYGRFGYGPATTLHELTVDRRFARFHADAPGGGLGGSSVRLVRPTEHRGEFEAIYERWRQQVPGGLLRPQVLWDELLAECKAAPGGDRESFALLHPDGYALYRVDRTDLKLARVSELRAVTADAHCALWRALIGLDSMERISIITHPQDPLPHLLTDTRLARTTWRQDGLWLRIMNVPAALEARGYAHEVGEFSTVLEVSDGGRFALKIGDGRARCTPTDAAAEIEMDRDVLGSLYLGAHRASTLAAANRLRTKDSQLLRRLDAAFASDVPVQTAFEF
Bibliography
- Wei J et al. [2000]. Identification of a Mycobacterium tuberculosis gene that enhances mycobacterial survival in macrophages. Mutant Product Function
- Rosenkrands I et al. [2000]. Towards the proteome of Mycobacterium tuberculosis. Proteomics
- Dahl JL et al. [2001]. Subcellular localization of the Iitracellular survival-enhancing Eis protein of Mycobacterium tuberculosis. Product Localization
- Vetting MW et al. [2005]. Structure and functions of the GNAT superfamily of acetyltransferases. Biochemistry
- Lella RK et al. [2007]. Eis (enhanced intracellular survival) protein of Mycobacterium tuberculosis disturbs the cross regulation of T-cells. Function
- Samuel LP et al. [2007]. Expression, production and release of the Eis protein by Mycobacterium tuberculosis during infection of macrophages and its effect on cytokine secretion. Function Mutant
- Zaunbrecher MA et al. [2009]. Overexpression of the chromosomally encoded aminoglycoside acetyltransferase eis confers kanamycin resistance in Mycobacterium tuberculosis. Mutant
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
