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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionInvolved in the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the inner membrane. Responsible for the translocation of the substrate across the membrane.
ProductProbable C4-dicarboxylate-transport transmembrane protein DctA
CommentsRv2443, (MTCY428.03c), len: 491 aa. Probable dctA, C4-dicarboxylate-transport transmembrane protein, similar to other C4-dicarboxylate transport proteins e.g. AAK46817|MT2519 from Mycobacterium tuberculosis strain CDC1551 (491 aa); Q9L1K8|SC6A11.12 putative sodium:dicarboxylate symporter from Streptomyces coelicolor (466 aa), FASTA scores: opt: 1797, E(): 2.9e-98, (61.3% identity in 452 aa overlap); Q9RRG7|DR2525 from Deinococcus radiodurans (463 aa); P50334|DCTA_SALTY from Salmonella typhimurium (428 aa) FASTA scores: opt: 1241, E(): 1.3e-65, (47.2% identity in 415 aa overlap); etc. Belongs to the sodium dicarboxylate symporter family (SDF) (DAACS family).
Functional categoryCell wall and cell processes
ProteomicsIdentified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS27407092742184+
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2443|dctA
MTAPLDRAPVTDLPANNKGRDRTHWLYLAVIFAVIAGVIVGLTAPSTGKSLTVLGTVFVNLIKMMIAPVIFCTIVLGIGSVRKAAAVGKVGGLALAYFLTMSSVALGIGLIVGNLLSPGRDLHLRPGAVGSGAALAGQAAESHGIAGFIQQIIPRSLPSALTEGNVLQVLLVALLVGFAVQGLGPAGESILRAVENLQKLVFKVLVMVLWLAPIGAFGAIANIVATTGFNAVTNLLLLMAGFYLTCVVFVFGVLGVLLRIVSGLSIFRLLRYLAREYLLIFATSSSEVVLPRLITKMKHLGVQSSTVGVVVPTGYSFNLDGTAIYLTMASLFIADAMGHRLTWGEQIALLAFMIIASKGAAGVSGAGLATLAGGLQAHRPELLDGVGLIVGIDRFMSEARSLTNFSGNAVATILVASWTKTIDLSKADEVLRGRDPFDESTMVDPHDEEPPAATPHGGGVPTNPALCDFEQVSLGGLVGRPAGPQRADVDG