Gene Rv2485c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; lipolytic enzyme involved in cellular metabolism. |
| Product | Probable carboxylesterase LipQ |
| Comments | Rv2485c, (MTV008.41c), len: 421 aa. Probable lipQ, carboxylesterase protein (lipase). Similar (greater at the C-terminal end) to AAK46626|MT2342 putative carboxylesterase from Mycobacterium tuberculosis strain CDC1551 (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60, (46.25% identity in 428 aa overlap); and Q50681|Rv2284|MTCY339.26c hypothetical protein from M. tuberculosis strain H37Rv (431 aa), FASTA scores: opt: 1134, E(): 4.3e-60, (46.25% identity in 428 aa overlap). Also similar in part to other putative lipases/esterases e.g. AAK44451|MT0230 from Mycobacterium tuberculosis strain CDC1551 (403 aa), FASTA scores: opt: 763, E(): 4.6e-38, (37.95% identity in 390 aa overlap); Q9RY19|DR0133 from Deinococcus radiodurans (296 aa), FASTA scores: opt: 392, E(): 4e-16, (33.7% identity in 276 aa overlap); Q9Z545|SC9B2.14 from Streptomyces coelicolor (502 aa) FASTA scores: opt: 279, E(): 3.2e-09, (31.15% identity in 292 aa overlap); etc. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Transcriptomics | DNA microarrays show lower level of expression in M. tuberculosis H37Rv than in Rv3676 mutant (See Rickman et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2792723 | 2793988 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2485c|lipQ
MHIASVTSRCSRAGAEALRQGAQLAADARDTCRAGALLLRGSPCAIGWVAGWLSAEFPARVVTGHALSRISPRSIGRFGTSWAAQRADQILHAALVDAFGPDFRDLVWHPTGEQSEAARRSGLLNLPHIPGPHRRYAAQTSDIPYGPGGRENLLDIWRRPDLAPGRRAPVLIQVPGGAWTINGKRPQAYPLMSRMVELGWICVSINYSKSPRCTWPAHIVDVKRAIAWVRENIADYGGDPDFITITGGSAGAHLAALAALSANDPALQPGFESADTAVQAAAPYYGVYDLTNAENMHEMMMPFLEHFVMRSRYVDNPGLFKAASPISYVHSEAPPFFVLHGEKDPMVPSAQSRAFSAALRDAGAATVSYAELPNAHHAFDLAATVRSRMVAEAVSDFLGVIYGRRMGARKGSLALSSPPAS
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Rickman L, Scott C, Hunt DM, Hutchinson T, Menendez MC, Whalan R, Hinds J, Colston MJ, Green J and Buxton RS [2005]. A member of the cAMP receptor protein family of transcription regulators in Mycobacterium tuberculosis is required for virulence in mice and controls transcription of the rpfA gene coding for a resuscitation promoting factor. Transcriptome
- Deb C, Daniel J, Sirakova TD, Abomoelak B, Dubey VS and Kolattukudy PE [2006]. A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis. Product Transcriptome
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
