Gene Rv2488c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in transcriptional mechanism. |
| Product | Probable transcriptional regulatory protein (LuxR-family) |
| Comments | Rv2488c, (MTV008.44c), len: 1137 aa. Probable transcriptional regulatory protein, belonging to luxR family, similar to many in Mycobacterium tuberculosis e.g. AAK44621|MT0399 from strain CDC1551 (1092 aa) FASTA scores: opt: 3767, E(): 1.8e-211, (56.75% identity in 1093 aa overlap); O53720|Rv0386|MTV036.21 from strain H37Rv (1085 aa), FASTA scores: opt: 3756, E(): 7.6e-211, (56.75% identity in 1089 aa overlap); AAK45665|MT1402 from strain CDC1551 (1159 aa), FASTA scores: opt: 3395, E(): 8.2e-190, (52.0% identity in 1093 aa overlap); etc. Also similar to transcriptional regulatory proteins luxR-family from other organisms e.g. Q9CBP3|ML1753 from Mycobacterium leprae (1106 aa), FASTA scores: opt: 2823, E(): 1.5e-156, (50.35% identity in 1116 aa overlap); Q9KYF4|SCD72A.02 from Streptomyces coelicolor (1114 aa), FASTA scores: opt: 915, E(): 1.7e-45, (30.7% identity in 1143 aa overlap); etc. Some similarity with Q9KXP6|SC9C5.28 hypothetical 81.8 KDA protein from Streptomyces coelicolor (750 aa), FASTA scores: opt: 1085, E(): 1.6e-55, (35.45% identity in 722 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), PS00622 Bacterial regulatory proteins, luxR family signature, probable coiled-coil from aa 585 to 616 and probable helix-turn-helix motif at aa 1086 to 1107 (score 1206, +3.29 SD). Belongs to the LuxR/UhpA family of transcriptional regulators. |
| Functional category | Regulatory proteins |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 90 days but not 30 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2797467 | 2800880 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2488c|Rv2488c
MDRRPRDFEQSRRRCRCNALRAGSMLASMSKIHPGVDVVPVDWSADGVSELVPTGTVTLLLADIEGATHLPGSQLDTTAIAKLDRTLTELVREHRGVCPVEQGEGDSFLVAFARASDAVACALGLQRAPLAPIRLRIGMHTGEVSSPDEGNCVGPTIDRTARLRELAHGGQTVLSGTTSDLVADLLPKDAWLNDLGTYRLDDLPRPERVVQLCHPDLHNAFPPLRTRKVVGAHCLPAQLTRLVGRVDEVAQVRGLLDVKRWVTLTGVGGVGKTRLATQVASAVADGYPDGVWYVNLAPITDPALVPIAAARVLGLPDQPGRSTVDTIVRRIGDRRMLVVLDNCEHLLDGCAALIVALLGACPALRVLATSREPIAVAGEQIWRVPPLGHGEAIELFTDRAREARPELEITADNLALVTEICHRLDGIPLAIELAASRVRALALTEIVDSLHDRFRLLTGGSRIAVRRQQTMRASVDWSHALLTGPEQVLFRRLAVFPSGFDLDGAQAAAAGGDVQRYEVVDLLSLLADKSLVVTDDSDGRTRYRLLETVRQYALEKLRESGDADAVRARHRDHYAAVAAGLDAPSVAGHERRLNQAELEIDNLRAAFAFSRENGDTGHALLLASCLQPLWRARGRLQEGLAWFAAALADHDAHPAGADPGLYARALADRALIDAVAGITDRLDDAQKALAIARDIEDPALLARALTACGGVAAYNADLARPWLAEAVGLARAVGDKWRLAEVLAWQAYVGFAGEGDPGATRAAGEEARSLADEIGDAFLSRSCRWALAAANLWQGNLEAAVGLSREVIGESDAAHDMVSSCAGQACLAHALAHRGDTEAAAAAQASIDTAVGLSPVLSGSACSALVFATLAAGDVAAAEHARESATRFFGASAAAIINDPTSSAQISCARGDLNAAHRLADGAASITRGVHRARALTTRCRIEIAQGDRHRAERDAHDALGVAASIGAYLWVPDILECLASVMADAGSNREAVRLFGAADAARGRMGAVRFGIYQAGCNSSLATLRKSMGDSEFDDAWAEGTALSIDEAIAYAQRGRGARKRPTSGWGALTPTELEVALLVGEGLSNKEIGVRLFISPRTVHSHLTHVYTKLGLSSRLQLAQQAARRGESERGPSRP
Bibliography
- Parish T, Smith DA, Roberts G, Betts J and Stoker NG [2003]. The senX3-regX3 two-component regulatory system of Mycobacterium tuberculosis is required for virulence. Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
