Gene Rv2498c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Interconversion of acetate and oxaloacetate from citrate [catalytic activity: citrate = acetate + oxaloacetate]. |
| Product | Probable citrate (pro-3S)-lyase (beta subunit) CitE (citrase) (citratase) (citritase) (citridesmolase) (citrase aldolase) |
| Comments | Rv2498c, (MTCY07A7.04c), len: 273 aa. Probable citE, citrate lyase, beta subunit, similar to others e.g. Q9S3L3|cite from Corynebacterium glutamicum (Brevibacterium flavum) (217 aa), FASTA scores: opt: 565, E(): 1.5e-28, (41.85% identity in 215 aa overlap); Q9HRM8|cite|VNG0627G from Halobacterium sp. strain NRC-1 (303 aa), FASTA scores: opt: 535, E(): 1.5e-26, (41.65% identity in 276 aa overlap); Q9S2U9|SC4G6.02 from Streptomyces coelicolor (274 aa), FASTA scores: opt: 426, E(): 1e-19, (37.6% identity in 274 aa overlap); P77770|CILB_ECOLI from Escherichia coli (307 aa), FASTA scores: opt: 265, E(): 1.5e-10, (32.8% identity in 265 aa overlap); etc. Also similar to Rv3075c|MTCY22D7.06 from Mycobacterium tuberculosis, FASTA score: (35.2% identity in 264 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2812355 | 2813176 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2498c|citE
MNLRAAGPGWLFCPADRPERFAKAAAAADVVILDLEDGVAEAQKPAARNALRDTPLDPERTVVRINAGGTADQARDLEALAGTAYTTVMLPKAESAAQVIELAPRDVIALVETARGAVCAAEIAAADPTVGMMWGAEDLIATLGGSSSRRADGAYRDVARHVRSTILLAASAFGRLALDAVHLDILDVEGLQEEARDAAAVGFDVTVCIHPSQIPVVRKAYRPSHEKLAWARRVLAASRSERGAFAFEGQMVDSPVLTHAETMLRRAGEATSE
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Goulding CW et al. [2007]. The structure and computational analysis of Mycobacterium tuberculosis protein CitE suggest a novel enzymatic function. Structure
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
