Gene Rv2503c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in various degradation and synthesis [catalytic activity: succinyl-CoA + a 3-oxo acid = succinate + a 3-oxo-acyl-CoA]. |
| Product | Probable succinyl-CoA:3-ketoacid-coenzyme A transferase (beta subunit) ScoB (3-oxo-acid:CoA transferase) (OXCT B) (succinyl CoA:3-oxoacid CoA-transferase) |
| Comments | Rv2503c, (MTCY07A7.09c, MT2578), len: 218 aa. Probable scoB, 3-oxo acid:CoA transferase, beta subunit (succinyl-CoA:3-ketoacid-CoA transferase). Highly similar to others e.g. Q9XAM8|SC4C6.12c from Streptomyces coelicolor (217 aa), FASTA scores: opt: 1048, E(): 2.6e-60, (73.9% identity in 207 aa overlap); Q9XD82|PCAJ from Streptomyces sp. 2065 (214 aa), FASTA scores: opt: 1031, E(): 3.2e-59, (70.8% identity in 209 aa overlap); AAK53493|LPSJ from Xanthomonas campestris (pv. campestris) (212 aa), FASTA scores: opt: 886, E(): 6.6e-50, (62.5% identity in 208 aa overlap); P42316|SCOB_BACSU from Bacillus subtilis (216 aa), FASTA scores: opt: 820, E(): 1.2e-45, (58.2% identity in 201 aa overlap); etc. Belongs to the 3-oxoacid CoA-transferase subunit B family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2818471 | 2819127 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2503c|scoB
MSAPGWSRDEMAARVAAEFEDGQYVNLGIGMPTLIPNHIPDGVHVVLHSENGILGVGPYPRREDVDADLINAGKETVTTLPGAAFFSSSTSFGIIRGGHLDVAVLGAMQVSVTGDLANWMIPGKMVKGMGGAMDLVHGARKVIVMMEHTAKDGSPKILERCTLPLTGVGCVDRIVTELAVIDVCADGLHLVQTAPGVSVDEVVAKTQPPLVLRDLATQ
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
