Gene Rv2565
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved protein |
| Comments | Rv2565, (MTCY9C4.03c), len: 583 aa. Conserved protein, similar in part to Q9A6C3|CC2171 hypothetical protein from Caulobacter crescentus (610 aa), FASTA scores: opt: 765, E(): 2.8e-37, (32.15% identity in 575 aa overlap). C-terminus also highly similar to various bacterial proteins e.g. O34731|YLBK_BACSU hypothetical 28.3 KDA protein from Bacillus subtilis (260 aa), FASTA scores: opt: 386, E(): 2.2e-15, (33.05% identity in 245 aa overlap); CAC45997|SMC01003 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (321 aa), FASTA scores: opt: 352, E(): 2.5e-13, (29.65% identity in 280 aa overlap); Q9K9Q8|BH2587 hypothetical protein from Bacillus halodurans (275 aa), FASTA scores: opt: 334, E(): 2.5e-12, (33.7% identity in 175 aa overlap); etc. And shows similarity to C-terminal half of some eukaryotic proteins e.g. Q9R114|NTE neuropathy target esterase homolog from Mus musculus (Mouse) (1327 aa), FASTA scores: opt: 411, E(): 2.7e-16, (24.45% identity in 626 aa overlap); O60859 neuropathy target esterase from Homo sapiens (Human) (1327 aa), FASTA scores: opt: 410, E(): 3.1e-16, (24.1% identity in 627 aa overlap); Q9U969|SWS|CG2212 swiss cheese protein from Drosophila melanogaster (Fruit fly) (1425 aa), FASTA scores: opt: 401, E(): 1.1e-15, (27.75% identity in 544 aa overlap); etc. Also shows strong similarity to C-terminal half of O05884|Z95121|Rv3239c|MTY20B11.14c hypothetical 110.2 KDA protein from Mycobacterium tuberculosis (1048 aa), FASTA scores: opt: 648, E(): 3e-30, (36.55% identity in 572 aa overlap); and O69695|Rv3728|MTV025.076 putative two-domain membrane protein from Mycobacterium tuberculosis (1065 aa), FASTA scores: opt: 643, E(): 6e-30, (34.3% identity in 595 aa overlap). Predicted to be an outer membrane protein (See Song et al., 2008). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2884611 | 2886362 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2565|Rv2565
MTTARRRPKRRGTDARTALRNVPILADIDDEQLERLATTVERRHVPANQWLFHAGEPADSIYIVDSGRFVAVAPEGHVFAEMASGDSIGDLGVIAGAARSAGVRALRDGVVWRIAAETFTDMLEATPLLQSAMLRAMARMLRQSRPAKTARRPRVIGVVSNGDTAAAPMVDAIATSLDSHGRTAVIAPPVETTSAVQEYDELVEAFSETLDRAERSNDWVLVVADRGAGDLWRHYVSAQSDRLVVLVDQRYPPDAVDSLATQRPVHLITCLAEPDPSWWDRLAPVSHHPANSDGFGALARRIAGRSLGLVMAGGGARGLAHFGVYQELTEAGVVIDRFGGTSSGAIASAAFALGMDAGDAIAAAREFIAGSDPLGDYTIPISALTRGGRVDRLVQGFFGNTLIEHLPRGFFSVSADMITGDQIIHRRGSVSGAVRASISIPGLIPPVHNGEQLLVDGGLLNNLPANVMCADTDGEVICVDLRRTFVPSKGFGLLPPIVTPPGLLRRLLTGTDNALPPLQETLLRAFDLAASTANLRELPRVAAIIEPDVSKIGVLNFKQIDAALEAGRMAARAALQAQPDLVR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Song H, Sandie R, Wang Y, Andrade-Navarro MA and Niederweis M [2008]. Identification of outer membrane proteins of Mycobacterium tuberculosis. Localization
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
