Gene Rv2567
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical alanine and leucine rich protein |
| Comments | Rv2567, (MTCY227.34c, MTCY9C4.01c), len: 884 aa. Conserved hypothetical ala-, leu-rich protein, equivalent to O53121|ML2679|MLCB1913.13 hypothetical protein from Mycobacterium leprae (893 aa), FASTA scores: opt: 4326, E(): 0, (75.2% identity in 883 aa overlap); and similar to Q49755|YO11_MYCLE|ML0605|MLCL536.05c|U1937B|B1937_F1_4 hypothetical 61.8 KDA protein from Mycobacterium leprae (561 aa), FASTA scores: opt: 758, E(): 1.2e-38, (32.2% identity in 537 aa overlap). Also similar to others e.g. Q9HUN7|PA4927 hypothetical protein from Pseudomonas aeruginosa (830 aa), FASTA scores: opt: 1247, E(): 2.2e-68, (38.25% identity in 831 aa overlap); Q98HG7|MLL2876 hypothetical protein from Rhizobium loti (Mesorhizobium loti) (803 aa), FASTA scores: opt: 937, E(): 1.9e-49, (32.15% identity in 828 aa overlap); CAC47419|SMC04057 conserved hypothetical protein from Rhizobium meliloti (Sinorhizobium meliloti) (802 aa), FASTA scores: opt: 900, E(): 3.4e-47, (30.85% identity in 852 aa overlap); etc. And similar to P71732|YO11_MYCTU|Rv2411c|MT2484|MTCY253.09 conserved hypothetical protein from Mycobacterium tuberculosis (551 aa), FASTA scores: opt: 781, E(): 4.6e-40, (33.75% identity in 495 aa overlap). |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in M. tuberculosis H37Rv-infected guinea pig lungs at 30 and 90 days (See Kruh et al., 2010). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2889795 | 2892449 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2567|Rv2567
MAPSASAATNGYDVDRLLAGYRTARAQETLFDLRDGPGAGYDEFVDDDGNVRPTWTELADAVAERGKAGLDRLRSVVHSLIDHDGITYTAIDAHRDALTGDHDLEPGPWRLDPLPLVISAADWEVLEAGLVQRSRLLDAILADLYGPRSMLTEGVLPPEMLFAHPGYVRAANGIQMPGRHQLFMHACDLSRLPDGTFQVNADWTQAPSGSGYAMADRRVVAHAVPDLYEELAPRPTTPFAQALRLALIDAAPDVAQDPVVVVLSPGIYSETAFDQAYLATLLGFPLVESADLVVRDGKLWMRSLGTLKRVDVVLRRVDAHYADPLDLRADSRLGVVGLVEAQHRGTVTVVNTLGSGILENPGLLRFLPQLSERLLDESPLLHTAPVYWGGIASERSHLLANVSSLLIKSTVSGETLVGPTLSSAQLADLAVRIEAMPWQWVGQELPQFSSAPTNHAGVLSSAGVGMRLFTVAQRSGYAPMIGGLGYVLAPGPAAYTLKTVAAKDIWVRPTERAHAEVITVPVLAPPAKTGAGTWAVSSPRVLSDLFWMGRYGERAENMARLLIVTRERYHVFRHQQDTDESECVPVLMAALGKITGYDTATGAGSAYDRADMIAVAPSTLWSLTVDPDRPGSLVQSVEGLALAAQAVRDQLSNDTWMVLANVERAVEHKSDPPQSLAEADAVLASAQAETLAGMLTLSGVAGESMVHDVGWTMMDIGKRIERGLWLTALLQATLSTVRHPAAEQAIIEATLVACESSVIYRRRTVGKFSVAAVTELMLFDAQNPRSLVYQLERLRADLKDLPGSSGSSRPERMVDEMNTRLRRSHPEELEEVSADGLRAELAELLAGIHASLRDVADVLTATQLALPGGMQPLWGPDQRRVMPA
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Kruh NA et al. [2010]. Portrait of a pathogen: the Mycobacterium tuberculosis proteome in vivo. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- Mazandu GK et al. [2012]. Function prediction and analysis of mycobacterium tuberculosis hypothetical proteins. Function
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
