Gene Rv2605c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; lipolytic enzyme involved in cellular metabolism. Can hydrolyze a broad range of acyl-CoA thioesters. |
| Product | Probable acyl-CoA thioesterase II TesB2 (TEII) |
| Comments | Rv2605c, (MTCY01A10.28), len: 281 aa. Probable tesB2, acyl-CoA thioesterase II, highly similar to others e.g. Q98EG9|MLL4250 from Rhizobium loti (Mesorhizobium loti) (286 aa), FASTA scores: opt: 563, E(): 3.9e-29, (47.75% identity in 287 aa overlap); CAC47767 from Rhizobium meliloti (Sinorhizobium meliloti) (294 aa), FASTA scores: opt: 553, E(): 1.8e-28, (49.3% identity in 280 aa overlap); P23911|TESB_ECOLI|B0452 from Escherichia coli strain K12 (285 aa), FASTA scores: opt: 487, E(): 3.1e-24, (41.9% identity in 277 aa overlap); etc. Also similar to O06135|TESB1|Rv1618|MTCY01B2.10 acyl-CoA thioesterase II from Mycobacterium tuberculosis (300 aa), FASTA scores: opt: 425, E(): 1.1e-21, (34.9% identity in 278 aa overlap). Belongs to the C/M/P thioester hydrolase family. |
| Functional category | Lipid metabolism |
| Proteomics | Identified by proteomics at the Statens Serum Institute (Denmark) (See Rosenkrands et al., 2000). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 2932297 | 2933142 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2605c|tesB2
VSIEEILDLEQLEVNIYRGSVFSPESGFLQRTFGGHVAGQSLVSAVRTVDPRYMVHSLHGYFLRPGDAKERTVFLVERIRDGGSFCTRRVNAVQHGETIFSMAASFQTEQEGITHQDVMPAAPPPDGLPGLNSIKVFDDAGFRQFDEWDVCIVPRERLRLLPGKASQQQVWLRHRDPLPDDPVLHICALAYMSDLTLLGSAQVNHLDVRDQLQVASLDHAMWFMRPFRADEWLLYDQSSPSASGGRALTRGEIFTRSGEMVAAVMQEGLTRHRRGHRSVGQ
Bibliography
- Rosenkrands I et al. [2000]. Towards the proteome of Mycobacterium tuberculosis. Proteomics
- Gao LY et al. [2003]. Transposon mutagenesis of Mycobacterium marinum identifies a locus linking pigmentation and intracellular survival. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- de Souza GA et al. [2011]. Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Proteomics Sequence
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
