Gene Rv2715
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible hydrolase |
| Comments | Rv2715, (MTCY05A6.36), len: 341 aa. Possible hydrolase, showing some similarity with other hydrolases e.g. Q9I5B0|PA0829 probable hydrolase from Pseudomonas aeruginosa (313 aa), FASTA scores: opt: 336, E(): 9.9e-14, (28.05% identity in 289 aa overlap); BAB55888 hydrolase (fragment) from Terrabacter sp. DBF63 (319 aa), FASTA scores: opt: 326, E(): 4.2e-13, (27.95% identity in 290 aa overlap); O52866|CEH|eh soluble epoxide hydrolase from Corynebacterium SP (285 aa), FASTA scores: opt: 325, E(): 4.4e-13, (29.95% identity in 284 aa overlap); etc. Also shows some similarity to P96811|EPHF|Rv0134|MTCI5.08 hypothetical 33.8 KDA protein from Mycobacterium tuberculosis (300 aa), FASTA scores: E(): 1.8e-10, (27.7% identity in 271 aa overlap). Contains lipases, serine active site motif (PS00120). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3028098 | 3029123 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2715|Rv2715
MTERKRNLRPVRDVAPPTLQFRTVHGYRRAFRIAGSGPAILLIHGIGDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDLLSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGSEAMALLRLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTLRAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDVVLPVRHAHMAHAAMPGSQLEIFEGSGHFPFHDDPARFIDIVERFMDTTEPAEYDQAALRALLRRGGGEATVTGSADTRVAVLNAIGSNERSAT
Bibliography
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
