Gene Rv2790c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly catalyzes the transfer of a great variety of lipids between membranes. |
| Product | Probable lipid-transfer protein Ltp1 |
| Comments | Rv2790c, (MTV002.55c), len: 401 aa. Probable ltp1, lipid-transfer protein, highly similar to many eukaryotic sterol-carrier proteins/lipid-transfer protein precursors (see Ossendorp & Wirtz 1993) e.g. O62742|SCP2 sterol carrier protein X from Oryctolagus cuniculus (Rabbit) (547 aa), FASTA scores: opt: 1710, E(): 6e-102, (63.7% identity in 394 aa overlap); Q9QW19 3-oxoacyl-CoA thiolase homolog (fragment) from Rattus sp. (405 aa), FASTA scores: opt: 1696, E(): 3.8e-101, (63.2% identity in 394 aa overlap); P11915|NLTP_RAT|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Rattus norvegicus (Rat) (547 aa), FASTA scores: opt: 1696, E(): 4.8e-101, (63.2% identity in 394 aa overlap); P32020|NLTP_MOUSE|SCP2|SCP-2 nonspecific lipid-transfer protein precursor from Mus musculus (Mouse) (547 aa), FASTA scores: opt: 1681, E(): 4.3e-100, (62.7% identity in 394 aa overlap); etc. Contains PS00098 Thiolases acyl-enzyme intermediate signature and PS00737 Thiolases signature 2. Also similar to other M. tuberculosis proteins e.g. O06144|Rv1627c|MTCY01B2.19c (402 aa) (35.8% identity in 413 aa overlap). |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the cell wall fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 24h of starvation (see Betts et al., 2002). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3098964 | 3100169 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2790c|ltp1
MPNQGSSNKVYVIGVGMTKFEKPGRREGWDYPDMARESGTKALRDAGIDYREVEQGYVGYVYGESTSGQRALYELGMTGIPIVNVNNNCSTGSTALYLGAQAIRGGLADCVLALGFEKMQPGALGGGADDRESPLGRHVKALAEIDEFGFPVAPWMFGAAGREHMKKYGTTAEHFAKIGYKNHKHSVNNPYAQFQDEYTLDDILASKMISDPLTKLQCSPTSDGSAAVVLASEDYLANHNLAGRAVEIVGQAMTTDFASTFDGSARNIIGYDMTVQAAQRVYQQSGLGPKDFGVIELHDCFSANELLLYEALGLCGPGEAPELIDDNQTTYGGRWVVNPSGGLISKGHPLGATGLAQCAELTWQLRGTAEARQVDNVTAALQHNIGLGGAAVVTAYQRAER
Bibliography
- Ossendorp BC et al. [1993]. The non-specific lipid-transfer protein (sterol carrier protein 2) and its relationship to peroxisomes. Secondary Review
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
