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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
PE/PPE
intermediary metabolism and respiration
unknown
regulatory proteins
conserved hypotheticals
lipid metabolism
pseudogenes
General annotation
TypeCDS
FunctionAssociates with the EF-TU.GDP complex and induces the exchange of GDP to GTP, it remains bound to the aminoacyl-tRNA. EF-TU.GTP complex up to the GTP hydrolysis stage on the ribosome.
ProductProbable elongation factor Tsf (EF-ts)
CommentsRv2889c, (MTCY274.20c), len: 271 aa. Probable tsf, elongation factor, equivalent to O33039|EFTS_MYCLE|TSF|ML1597|MLCB250.64 elongation factor from Mycobacterium leprae (276 aa), FASTA scores: opt: 1430, E(): 1.9e-80, (83.7% identity in 276 aa overlap). Also highly similar to others e.g. Q9X5Z9|EFTS_STRRA|TSF from Streptomyces ramocissimus (278 aa), FASTA scores: opt: 928, E(): 1.1e-49, (57.05% identity in 277 aa overlap); O31213|EFTS_STRCO|TSF|SC2E1.42 from Streptomyces coelicolor (278 aa), FASTA scores: opt: 927, E(): 1.3e-49, (56.3% identity in 277 aa overlap); P80700|EFTS_BACSU|TSF from Bacillus subtilis (292 aa), FASTA scores: opt: 650, E(): 1.3e-32, (43.85% identity in 276 aa overlap); etc. Contains PS01127 Elongation factor Ts signature 2. Belongs to the EF-ts family.
Functional categoryInformation pathways
ProteomicsThe product of this CDS corresponds to spot 3_487 identified in culture supernatant by proteomics at the Max Planck Institute for Infection Biology, Berlin, Germany (see citations below). Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS (See Gu et al., 2003). Identified in the culture supernatant of M. tuberculosis H37Rv using mass spectrometry (See Mattow et al., 2003). Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified in the detergent phase of Triton X-114 extracts of M. tuberculosis H37Rv membranes using 1-DGE and MALDI-TOF-MS (See Sinha et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the culture filtrate, membrane protein fraction, and whole cell lysates of M. tuberculosis H37Rv (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011).
MutantEssential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Coordinates
TypeStartEndOrientation
CDS31982923199107-
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
       
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2889c|tsf
MANFTAADVKRLRELTGAGMLACKNALAETDGDFDKAVEALRIKGAKDVGKRAERATAEGLVAAKDGALIELNCETDFVAKNAEFQTLADQVVAAAAAAKPADVDALKGASIGDKTVEQAIAELSAKIGEKLELRRVAIFDGTVEAYLHRRSADLPPAVGVLVEYRGDDAAAAHAVALQIAALRARYLSRDDVPEDIVASERRIAEETARAEGKPEQALPKIVEGRLNGFFKDAVLLEQASVSDNKKTVKALLDVAGVTVTRFVRFEVGQA
      
Bibliography