Gene Rv2893
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown; probably involved in cellular metabolism. |
| Product | Possible oxidoreductase |
| Comments | Rv2893, (MTCY274.24), len: 325 aa. Possible oxidoreductase, showing similarity with various proteins and/or oxidoreductases e.g. Q9AE05|RIF11 eleventh protein in the rif biosynthetic gene cluster from Amycolatopsis mediterranei (Nocardia mediterranei) (294 aa), FASTA scores: opt: 270, E(): 4.8e-10, (34.5% identity in 313 aa overlap); O52567 reductase from Amycolatopsis mediterranei (Nocardia mediterranei) (153 aa), FASTA scores: opt: 251, E(): 5e-09, (42.4% identity in 125 aa overlap); Q58929|mer|MJ1534 F420-dependent methylenetetrahydromethanopterin reductase from Methanococcus jannaschii (331 aa), FASTA scores: opt: 249, E(): 1.2e-08, (29.7% identity in 283 aa overlap); etc. Also some similarity with others proteins from Mycobacterium tuberculosis strains H37Rv and CDC1551 e.g. P71844|Rv0791c|MTCY369.35c putative oxidoreductase (347 aa), FASTA scores: opt: 264, E(): 1.3e-09, (29.05% identity in 272 aa overlap); and P96809|Rv0132|MTCI5.06c putative oxidoreductase (360 aa), FASTA scores: opt: 260, E(): 2.4e-09, (33.05% identity in 239 aa overlap). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cytosol of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Transcriptomics | mRNA identified by microarray analysis; transcription repressed at low pH in vitro conditions, which may mimic an environmental signal encountered by phagocytosed bacteria (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3202420 | 3203397 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2893|Rv2893
MTVASTAHHTRRLRFGLAAPLPRAGTQMRAFAQAVEAAGFDVLAFPDHLVPSVSPFAGATAAAMATQRLHTGTLVLNNDFRHPVDTAREAAGVATLAEGRFELGLGAGHRRSEYDAAGITFDSGATRVARLIESAHLIRALLDAEPVDFDGQHYRVHAEAGSLVAPPKVRVPLLVGGNGTEVLRLGGRIADIVGLAGISHNRDATQVRFTHFDADGLADRIAVVRHAAGDRFEAIELNALIQAVVCTNDRNAAAAELAATLGGITPEQVLESPFLLLGTHEQMAEALAARQRRFGVSYWTVFDEWAGRASAMRDIAEVIALLRYG
Bibliography
- Fisher MA, Plikaytis BB and Shinnick TM [2002]. Microarray analysis of the Mycobacterium tuberculosis transcriptional response to the acidic conditions found in phagosomes. Transcriptome Regulation
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
