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virulence, detoxification, adaptation
information pathways
cell wall and cell processes
stable RNAs
insertion seqs and phages
intermediary metabolism and respiration
regulatory proteins
conserved hypotheticals
lipid metabolism
General annotation
FunctionThought to be involved in intracellular removal of iron from iron-mycobactin complex. Mycobactin is an iron-chelating compound involved in the transport of iron from the bacterial environment into the cell cytoplasm.
ProductPossible mycobactin utilization protein ViuB
CommentsRv2895c, (MT2963, MTCY274.26c), len: 283 aa. Possible viuB, mycobactin utilization protein, highly similar to Q9RJ78|SCI41.06 hypothetical 31.5 KDA protein from Streptomyces coelicolor (280 aa), FASTA scores: opt: 639, E(): 5.1e-32, (46.3% identity in 285 aa overlap); and similar to other proteins e.g. Q9F641|MXCB protein of the biosynthetic gene cluster of the myxochelin-type iron chelator from Stigmatella aurantiaca (270 aa), FASTA scores: opt: 417, E(): 2.2e-18, (34.2% identity in 263 aa overlap); Q56646|VIUB_VIBCH|VC2210 vibriobactin utilization protein from Vibrio cholerae (271 aa), FASTA scores: opt: 395, E(): 5.1e-17, (31.0% identity in 274 aa overlap); Q56743|VIUB_VIBVU vulnibactin utilization protein V from Vibrio vulnificus (271 aa), FASTA scores: opt: 390, E(): 1e-16, (33.95% identity in 274 aa overlap); etc. Equivalent to AAK47289 from Mycobacterium tuberculosis strain CDC1551 (321 aa) but shorter 38 aa.
Functional categoryIntermediary metabolism and respiration
ProteomicsIdentified in the culture supernatant of M. tuberculosis H37Rv using mass spectrometry (See Mattow et al., 2003). Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by Western blot in the cytoplasmic membrane fraction of M. tuberculosis H37Rv and not the cell wall or cytosol (See Farhana et al., 2008). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). Translational start site supported by proteomics data (See de Souza et al., 2011) (See Kelkar et al., 2011).
MutantNon-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011).
Check for mutants available at TARGET website
Genomic sequence
Feature type Upstream flanking region (bp) Downstream flanking region (bp) Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2895c|viuB