Gene Rv2921c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Probably involved in the reception and insertion of a subset of proteins at the membrane: possibly membrane receptor for FFH|Rv2916c. |
| Product | Probable cell division protein FtsY (SRP receptor) (signal recognition particle receptor) |
| Comments | Rv2921c, (MTCY338.10c, MT2989), len: 422 aa. Probable ftsY, signal recognition particle (SRP) receptor, a membrane-associated cell division protein (see citation below), equivalent to O33010|FTSY_MYCLE cell division protein FTSY homolog from Mycobacterium leprae (430 aa), FASTA scores: opt: 1760, E(): 1.1e-108, (81.35% identity in 429 aa overlap). Also similar to others e.g. Q9I6C1|FTSY|PA0373 signal recognition particle receptor FTSY from Pseudomonas aeruginosa (455 aa), FASTA scores: opt: 882, E(): 5.1e-40, (42.08% identity in 385 aa overlap); Q9KVJ6|FTSY cell division protein from Vibrio cholerae (391 aa), FASTA scores: opt: 837, E(): 1.2e-37, (36.3% identity in 394 aa overlap); P10121|FTSY_ECOLI|FTSY|B3464 cell division protein from Escherichia coli strain K12 (497 aa), FASTA scores: opt: 800, E(): 1.3e-35, (39.75% identity in 327 aa overlap); etc. Also similar to Q9ZBP9|SC7A1.24 putative prokaryotic docking protein from Streptomyces coelicolor (412 aa), FASTA scores: opt: 1461, E(): 4.3e-71, (60.3% identity in 423 aa overlap). Contains PS00017 ATP/GTP-binding site motif A (P-loop), and PS00300 SRP54-type proteins GTP-binding domain signature. Belongs to the SRP family of GTP-binding proteins. |
| Functional category | Cell wall and cell processes |
| Proteomics | Identified in the membrane fraction of M. tuberculosis H37Rv using 1D-SDS-PAGE and uLC-MS/MS; predicted transmembrane protein (See Gu et al., 2003). Identified in the membrane fraction of M. tuberculosis H37Rv using nanoLC-MS/MS (See Xiong et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Required for growth in C57BL/6J mouse spleen, by transposon site hybridization (TraSH) in H37Rv (See Sassetti and Rubin, 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3232871 | 3234139 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv2921c|ftsY
VWEGLWIATAVIAALVVIAALTLGLVLYRRRRISLSPRPERGVVDRSGGYTASSGITFSQTPTTQPAERIDTSGLPAVGDDATVPRDAPKRTIADVHLPEFEPEPQAPEVPEADAIAPPEGRLERLRGRLARSQNALGRGLLGLIGGGDLDEDSWQDVEDTLLVADLGPAATASVVSQLRSRLASGNVRTEADARAVLRDVLINELQPGMDRSIRALPHAGHPSVLLVVGVNGTGKTTTVGKLARVLVADGRRVVLGAADTFRAAAADQLQTWAARVGAAVVRGPEGADPASVAFDAVDKGIAAGADVVLIDTAGRLHTKVGLMDELDKVKRVVTRRASVDEVLLVLDATIGQNGLAQARVFAEVVDISGAVLTKLDGTAKGGIVFRVQQELGVPVKLVGLGEGPDDLAPFEPAAFVDALLG
Bibliography
- Braunstein M and Belisle JT [2000]. Review
- Sassetti CM and Rubin EJ [2003]. Genetic requirements for mycobacterial survival during infection. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Gu S et al. [2003]. Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain. Proteomics
- Xiong Y, Chalmers MJ, Gao FP, Cross TA and Marshall AG [2005]. Identification of Mycobacterium tuberculosis H37Rv integral membrane proteins by one-dimensional gel electrophoresis and liquid chromatography electrospray ionization tandem mass spectrometry. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
