Gene Rv3032
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in the synthesis of glycogen and 6-O-methylglucosyl-containing lipopolysaccharides (MGLP) |
| Product | Alpha (1->4) glucosyltransferase |
| Comments | Rv3032, (MTV012.47), len: 414 aa. Alpha (1->4) glucosyltransferase (See Stadthagen et al., 2007). Equivalent to Q9CBR3|ML1715 putative transferase from Mycobacterium leprae (438 aa), FASTA scores: opt: 2456, E(): 7.3e-145, (87.9% identity in 414 aa overlap). Also similar to hypothetical proteins and various transferases e.g. P73369|SLL1971 hypothetical 46.2 KDA protein from Synechocystis sp. strain PCC 6803 (404 aa), FASTA scores: opt: 584, E(): 7.3e-29, (34.5% identity in 400 aa overlap); Q9Z5B7|SC2G5.06 putative transferase from Streptomyces coelicolor (406 aa), FASTA scores: opt: 509, E(): 3.3e-24, (35.9% identity in 413 aa overlap); Q9UZA1|PAB0827 galactosyltransferase (LPS biosynthesis RFBU related protein) from Pyrococcus abyssi (371 aa), FASTA scores: opt: 381, E(): 2.6e-16, (26.75% identity in 404 aa overlap); etc. |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Disruption of this gene results in growth defect of H37Rv in vitro, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Mutation of Rv3032 in M. tuberculosis H37Rv has no effect on glucan content; mutant produces less glycogen; growth in BALB/c mice is comparable to wild-type (See Sambou et al., 2008). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3391534 | 3392778 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3032|Rv3032
MRILMVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRCPSGTDPSTHPSSDEVTEGVRVIAAAQDPHEFTFGNDMMAWTLAMGHAMIRAGLRLKKLGTDRSWRPDVVHAHDWLVAHPAIALAQFYDVPMVSTIHATEAGRHSGWVSGALSRQVHAVESWLVRESDSLITCSASMNDEITELFGPGLAEITVIRNGIDAARWPFAARRPRTGPAELLYVGRLEYEKGVHDAIAALPRLRRTHPGTTLTIAGEGTQQDWLIDQARKHRVLRATRFVGHLDHTELLALLHRADAAVLPSHYEPFGLVALEAAAAGTPLVTSNIGGLGEAVINGQTGVSCAPRDVAGLAAAVRSVLDDPAAAQRRARAARQRLTSDFDWQTVATATAQVYLAAKRGERQPQPRLPIVEHALPDR
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Stadthagen G et al. [2007]. Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis. Function
- Sambou T et al. [2008]. Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: biosynthesis and impact on the persistence in mice. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
