Gene Rv3068c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | This enzyme participates in both the breakdown and synthesis of glucose [catalytic activity: alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate]. |
| Product | Probable phosphoglucomutase PgmA (glucose phosphomutase) (PGM) |
| Comments | Rv3068c, (MTCY22D7.13), len: 547 aa. Probable pgmA, phosphoglucomutase, highly similar to other phosphoglucomutases e.g. Q9L117|PGM from Streptomyces coelicolor (546 aa), FASTA scores: opt: 2569, E(): 2.8e-149, (71.4% identity in 545 aa overlap); Q9ABY5|CC0085 from Caulobacter crescentus (545 aa), FASTA scores: opt: 2465, E(): 6.2e-143, (70.4% identity in 541 aa overlap); P38569|PGMU_ACEXY|CELB from Acetobacter xylinum (555 aa), FASTA scores: opt: 2206, E(): 4e-127, (62.25% identity in 543 aa overlap); P74643|PGM|SLL0726 from Synechocystis sp. strain PCC 6803 (567 aa), FASTA scores: opt: 2168, E(): 8.5e-125, (60.0% identity in 550 aa overlap); P36938|PGMU_ECOLI|PGM|B0688 from Escherichia coli (546 aa), FASTA scores: opt: 2111, E(): 2.5e-121, (58.2% identity in 550 aa overlap). Also similar to other phosphomannomutases. Has phosphoglucomutase and phosphomannomutase signature (PS00710) and ATP/GTP-binding site motif A (P-loop) (PS00017). Belongs to the phosphohexose mutases family. |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3431979 | 3433622 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3068c|pgmA
MVANPRAGQPAQPEDLVDLPHLVTAYYSIEPDPDDLAQQVAFGTSGHRGSALTGTFNELHILAITQAIVEYRAAQGTTGPLFIGRDTHGLSEPAWVSALEVLAANQVVAVVDSRDRYTPTPAISHAILTYNRGRTEALADGIVVTPSHNPPSDGGIKYNPPNGGPADTAATTAIAKRANEILLARSMVKRLPLARALRTAQRHDYLGHYVDDLPNVVDIAAIREAGVRIGADPLGGASVDYWGEIAHRHGLDLTVVNPLVDATWRFMTLDTDGKIRMDCSSPDAMAGLIRTMFGNRERYQIATGNDADADRHGIVTPDEGLLNPNHYLAVAIEYLYTHRPSWPAGIAVGKTVVSSSIIDRVVAGIGRQLVEVPVGFKWFVDGLIGATLGFGGEESAGASFLRRDGSVWTTDKDGIIMALLAAEILAVTGATPSQRYHALAGEYGGPCYARIDAPADREQKARLARLSADQVSATELAGEPITAKLTTAPGNGAALGGLKVTTANAWFAARPSGTEDVYKIYAESFRGPQHLVEVQQTAREVVDRVIG
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
