Gene Rv3081
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3081, (MTV013.02), len: 412 aa. Conserved hypothetical protein. Second part of the protein (approximately residues 250-412) shares weak similarity with other hypothetical proteins e.g. Q9YEU3|APE0488 from Aeropyrum pernix (188 aa), FASTA scores: opt: 149, E(): 0.019, E(): 0.019, (29.5% identity in 173 aa overlap); and first part shares weak similarity with C-terminal part of Q9RVT9|DR0933 alpha-amlyase from Deinococcus radiodurans (644 aa), FASTA scores: opt: 127, E(): 1.4, (27.25% identity in 198 aa overlap). Equivalent to AAK47502|MT3166 hypothetical 48.3 KDA protein from Mycobacterium tuberculosis strain CDC1551 (436 aa) but shorter 24 aa in N-terminus. Contains PS00850 Glycine radical signature and possible helix-turn-helix motif at aa 53-74. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3446040 | 3447278 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3081|Rv3081
VTPHYRQAAASRLDTHRTQKLRSQTNGGKDRHQLTYEQFARMLTLMGPSDLWTVERAARHWGVSASRARAILSSRHIHRVSGYPAQAIKAVTLRQGARTDLKTANHLVPAAQAFTMAETGAAIGETEDERARLRIFFEFLRGADETGTSALDLIVDEPALIGEHRFDALLAAAAEYISARWGRPGPLWSVSIERFLDTAWWVSDLPSARAFAAVWTPAPFRRRGIYLDRHDLTSDGVCVMPEPVFNRTELQRAFTALAAKLERRGVVGQVHVVGGAAMLLAYNSRVTTRDIDALFSTDGPMLEAIREVADEMGWPRTWLNNQASGYVSRTPGEGAPVFDHPFLHVVATPAQHLLAMKVVAARGVRDGEDIRLLLDRLRITSAAGVWEIVARYFPAETITDRSRLLVEDLLNQ
Bibliography
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
