Gene Rv3094c
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Function unknown |
| Product | Conserved hypothetical protein |
| Comments | Rv3094c, (MTCY164.05c), len: 376 aa. Conserved hypothetical protein, some similarity with various proteins e.g. Q9RMR9|NRGC NRGC protein (corresponding gene seems regulated by NifA) from Bradyrhizobium japonicum (388 aa), FASTA scores: opt: 677, E(): 5.8e-35, (34.55% identity in 353 aa overlap); P26698|PIGM_RHOSO pigment protein from Rhodococcus sp. strain ATCC 21145 (387 aa), FASTA scores: opt: 480, E(): 1.2e-22, (28.7% identity in 376 aa overlap); Q9F0J3|NCNH hydroxylase from Streptomyces arenae (405 aa), FASTA scores: opt: 441, E(): 3.3e-20, (29.25% identity in 352 aa overlap); etc. Equivalent to AAK47516 from Mycobacterium tuberculosis strain CDC1551 (395 aa) but N-terminus shorter 19 aa. |
| Functional category | Conserved hypotheticals |
| Proteomics | Identified by mass spectrometry in whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate or membrane protein fraction (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3462761 | 3463891 | - |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3094c|Rv3094c
VNQSETEIEILAEKIARWARARSAEIERDRRLPDELVTRLREAGLLRATMPREVAAPELAPGRALRCAEAVARGDASAGWCVSIAITSALLVAYLPARSREEMFGGGRGVAAGVWAPRGTARSVDGGVVVSGRWPFCSGINHADIMFAGCFVDDRQVPSVVALNKDELQVLDTWHTLGLRGTGSHDCVADDVFVPADRVFSVFDGPIVDRPLYRFPVFGFFALSIGAAALGNARAAIDDLVELAGGKKGLGSTRTLAERSATQAAAATAESALGAARALFYEVIEAAWQVSHDAEAVPVTMRNRLRLAATHAVRTSADVVRSMYDLAGGTAIYDNAPLQRRFRDAFTATAHFQVNEASRELPGRVLLDQPADVSML
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
