Gene Rv3109 (moaA)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in molybdenum cofactor biosynthesis; involved in the biosynthesis of molybdopterin precursor Z from guanosine. |
| Product | Probable molybdenum cofactor biosynthesis protein A MoaA1 |
| Comments | Rv3109, (MTCY164.19), len: 359 aa. Probable moaA1, molybdenum cofactor biosynthesis protein, highly similar to others e.g. P39757|MOAA_BACSU|NARA|NARAB from Bacillus subtilis (341 aa), FASTA scores: opt: 810, E(): 6.2e-44, (39.75% identity in 327 aa overlap); O67929|MOAA_AQUAE|AQ_2183 from Aquifex aeolicus (320 aa), FASTA scores: opt: 794, E(): 6e-43, (40.55% identity in 323 aa overlap); Q9ZIM6|MOAA_STACA from Staphylococcus carnosus (340 aa), FASTA scores: opt: 783, E(): 3.2e-42, (38.65% identity in 326 aa overlap); etc. Also highly similar to O53143|MOAA3|MOA3_MYCTU|MT3427 molybdenum cofactor biosynthesis protein A 3 from Mycobacterium tuberculosis strain F4 (378 aa), FASTA scores: opt: 1762, E(): 4.7e-104, (74.3% identity in 350 aa overlap); and similar to O53881|MOA2_MYCTU|MOAA2|Rv0869c|MT0892|MTV043.62 molybdenum cofactor biosynthesis protein A 2 from Mycobacterium tuberculosis strain H37Rv (360 aa), FASTA scores: opt: 657, E(): 3e-34, (36.55% identity in 309 aa overlap). Belongs to the MoaA / NifB / PqqE family. Note that previously known as moaA. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Translational start site supported by proteomics data (See Kelkar et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3477649 | 3478728 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3109|moaA1
MSTPTLPDMVAPSPRVRVKDRCRRMMGDLRLSVIDQCNLRCRYCMPEEHYTWLPRQDLLSVKEISAIVDVFLSVGVSKVRITGGEPLIRPDLPEIVRTLSAKVGEDSGLRDLAITTNGVLLADRVDGLKAAGMKRITVSLDTLQPERFKAISQRNSHDKVIAGIKAVAAAGFTDTKIDTTVMRGANHDELADLIEFARTVNAEVRFIEYMDVGGATHWAWEKVFTKANMLESLEKRYGRIEPLPKHDTAPANRYALPDGTTFGIIASTTEPFCATCDRSRLTADGLWLHCLYAISGINLREPLRAGATHDDLVETVTTGWRRRTDRGAEQRLAQRERGVFLPLSTLKADPHLEMHTRGG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Mendoza Lopez P et al. [2010]. Characterization of the transcriptional regulator Rv3124 of Mycobacterium tuberculosis identifies it as a positive regulator of molybdopterin biosynthesis and defines the functional consequences of a non-synonymous SNP in the Mycobacterium bovis BCG orthologue. Regulon
- Kelkar DS et al. [2011]. Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Proteomics Sequence
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
