Gene Rv3112 (moaD)
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in molybdenum cofactor biosynthesis. |
| Product | Probable molybdenum cofactor biosynthesis protein D MoaD1 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1) |
| Comments | Rv3112, (MTCY164.22), len: 83 aa. Probable moaD1, molybdenum cofactor biosynthesis protein (molybdopterin converting factor (subunit 1)), similar to others e.g. Q9HJF0|TA1019 from Thermoplasma acidophilum (85 aa), FASTA scores: opt: 144, E(): 0.0012, (31.7% identity in 82 aa overlap); BAB59710|TVG0556526 from Thermoplasma volcanium (90 aa), FASTA scores: opt: 144, E(): 0.0012, (31.7% identity in 82 aa overlap); P30748|MOAD_ECOLI|CHLA4|CHLM|B0784 from Escherichia coli strain K12 (81 aa), FASTA scores: opt: 116, E(): 0.11, (36.9% identity in 84 aa overlap); etc. N-terminus also highly similar to to O53375|GPHA|Rv3323c|MTV016.23c MOAD-MOAE fusion protein from Mycobacterium tuberculosis (221 aa), FASTA scores: opt: 333, E(): 2e-16, (65.05% identity in 83 aa overlap); and some similarity with Rv0868c|MTV043.61c|MOAD2 putative molybdenum cofactor biosynthesis protein D 2 (92 aa). Note that previously known as moaD. This region is a possible MT-complex-specific genomic island (See Becq et al., 2007). |
| Functional category | Intermediary metabolism and respiration |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Essential gene by Himar1 transposon mutagenesis in H37Rv strain (see Sassetti et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3479700 | 3479951 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3112|moaD1
MIKVNVLYFGAVREACDETPREEVEVQNGTDVGNLVDQLQQKYPRLRDHCQRVQMAVNQFIAPLSTVLGDGDEVAFIPQVAGG
Bibliography
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Becq J, Gutierrez MC, Rosas-Magallanes V, Rauzier J, Gicquel B, Neyrolles O and Deschavanne P [2007]. Contribution of horizontally acquired genomic islands to the evolution of the tubercle bacilli. Sequence
- Mendoza Lopez P et al. [2010]. Characterization of the transcriptional regulator Rv3124 of Mycobacterium tuberculosis identifies it as a positive regulator of molybdopterin biosynthesis and defines the functional consequences of a non-synonymous SNP in the Mycobacterium bovis BCG orthologue. Regulon
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
