Gene Rv3138
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Involved in cellular metabolism [catalytic activity: S-adenosyl-L-methionine + dihydroflavodoxin + [formate acetyltransferase]-glycine = 5'-deoxyadenosine + methionine + flavodoxin + [formate acetyltransferase]-glycine-2-YL radical]. |
| Product | Probable pyruvate formate lyase activating protein PflA (formate acetyltransferase activating enzyme) ([pyruvate formate-lyase] activating enzyme) |
| Comments | Rv3138, (MTCY03A2.20c), len: 362 aa. Probable pflA, pyruvate formate lyase activating protein, similar to other e.g. Q9V0N1|PAB1859 from Pyrococcus abyssi (348 aa), FASTA scores: opt: 926, E(): 1.1e-52, (39.95% identity in 343 aa overlap); O27446|MTH1395 from Methanobacterium thermoautotrophicum (335 aa), FASTA scores: opt: 909, E(): 1.3e-51, (42.2% identity in 327 aa overlap); O28939|AF1330 from Archaeoglobus fulgidus (336 aa), FASTA scores: opt: 884, E(): 5.6e-50, (42.0% identity in 319 aa overlap); etc. Also similar to O50099|PH1391 hypothetical 40.2 KDA protein from Pyrococcus horikoshii (348 aa), FASTA scores: opt: 934, E(): 3.3e-53, (40.5% identity in 343 aa overlap); and other hypothetical proteins. |
| Functional category | Intermediary metabolism and respiration |
| Transcriptomics | mRNA identified by microarray analysis and down-regulated after 4h of starvation (see citation below). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in H37Rv and CDC1551 strains (see Sassetti et al., 2003 and Lamichhane et al., 2003). Non-essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3504195 | 3505283 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3138|pflA
MSDPFTIATKHWHRLHDSRIQCDVCPRACKLHEGQRGLCFVRGRFDDQVKLTSYGRSSGFCVDPIEKKPLNHFLPGSATLSFGTAGCNLACKFCQNWDISKSREIDVLASRAAPADIARTAHELGCRSVAFTYNDPTIFWEYAADVADACHDQGIKAVAVTAGYMCPEPRAEFYRRVDAANVDLKAFTEDFYRKVCVSHLRNVLDTLAYLRHQTNVWLEITTLLIPGRNDSDAEVAAECRWIRENLGVDVPVHFTAFHPDYKMMDTPATPTATLTRAREIGIGEGLRFVYTGNVHDAVGGSTSCPGCRATVIVRDWYSIRHYALTEDGRCQACGYQMPGVYDGPAGHWGQRRLPLLTSLSRM
Bibliography
- Betts JC et al. [2002]. Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Transcriptome
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Sassetti CM et al. [2003]. Genes required for mycobacterial growth defined by high density mutagenesis. Mutant
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
