Gene Rv3170
in Mycobacterium tuberculosis H37Rv
General annotation
| Type | CDS |
| Function | Possibly catalyzes the oxidative deamination: oxidize on primary amines, and perhaps on secondary and tertiary amines [catalytic activity: RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)]. Must have important function in metabolism. Supposedly involved in stationary-phase survival. |
| Product | Probable flavin-containing monoamine oxidase AofH (amine oxidase) (MAO) |
| Comments | Rv3170, (MT3259, MTV014.14), len: 448 aa. Probable aofH, flavin-containing (mono)amine oxidase, equivalent to a predicted homologous protein from Mycobacterium smegmatis (see citation below), and similar to many eukaryotic monoamine oxidases e.g. P49253|AOF_ONCMY from Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (522 aa), FASTA scores: opt: 869, E(): 5.3e-44, (37.7% identity in 448 aa overlap); P21396|AOFA_RAT|MAOA from Rattus norvegicus (Rat) (526 aa), FASTA scores: opt: 839, E(): 3.2e-42, (37.45% identity in 446 aa overlap); Q99NA8|MAO-a from Cavia porcellus (Guinea pig) (506 aa), FASTA scores: opt: 836, E(): 4.6e-42, (37.0% identity in 446 aa overlap); P21398|AOFA_BOVIN from Bos taurus (Bovine) (527 aa), FASTA scores: opt: 806, E(): 2.8e-40, (37.0% identity in 446 aa overlap); P21397|AOFA_HUMAN (527 aa), FASTA scores: opt: 801, E(): 5.6e-40, (37.2% identity in 446 aa overlap); etc. Alternative start possible at position 3538487. Belongs to the flavin monoamine oxidase family. Cofactor: FAD (potential). |
| Functional category | Intermediary metabolism and respiration |
| Proteomics | Identified in the cytosol and cell membrane fraction of M. tuberculosis H37Rv using 2DLC/MS (See Mawuenyega et al., 2005). Identified by mass spectrometry in Triton X-114 extracts of M. tuberculosis H37Rv (See Malen et al., 2010). Identified by mass spectrometry in the membrane protein fraction and whole cell lysates of M. tuberculosis H37Rv but not the culture filtrate (See de Souza et al., 2011). |
| Mutant | Non-essential gene for in vitro growth of H37Rv in a MtbYM rich medium, by Himar1 transposon mutagenesis (see Minato et al. 2019). Non-essential gene for in vitro growth of H37Rv, by analysis of saturated Himar1 transposon libraries (see DeJesus et al. 2017). Non essential gene by Himar1 transposon mutagenesis in CDC1551 strain (see Lamichhane et al., 2003). Essential gene for in vitro growth of H37Rv, by Himar1 transposon mutagenesis (See Griffin et al., 2011). Check for mutants available at TARGET website |
Coordinates
| Type | Start | End | Orientation |
|---|---|---|---|
| CDS | 3538505 | 3539851 | + |
Genomic sequence
Feature type
Upstream flanking region (bp)
Downstream flanking region (bp)
Update
Protein sequence
>Mycobacterium tuberculosis H37Rv|Rv3170|aofH
VTNPPWTVDVVVVGAGFAGLAAARELTRQGHEVLVFEGRDRVGGRSLTGRVAGVPADMGGSFIGPTQDAVLALATELGIPTTPTHRDGRNVIQWRGSARSYRGTIPKLSLTGLIDIGRLRWQFERIARGVPVAAPWDARRARELDDVSLGEWLRLVRATSSSRNLMAIMTRVTWGCEPDDVSMLHAARYVRAAGGLDRLLDVKNGAQQDRVPGGTQQIAQAAAAQLGARVLLNAAVRRIDRHGAGVTVTSDQGQAEAGFVIVAIPPAHRVAIEFDPPLPPEYQQLAHHWPQGRLSKAYAAYSTPFWRASGYSGQALSDEAPVFITFDVSPHADGPGILMGFVDARGFDSLPIEERRRDALRCFASLFGDEALDPLDYVDYRWGTEEFAPGGPTAAVPPGSWTKYGHWLREPVGPIHWASTETADEWTGYFDGAVRSGQRAAAEVAALL
Bibliography
- Keer J, Smeulders MJ, Gray KM and Williams HD [2000]. Mutants of Mycobacterium smegmatis impaired in stationary-phase survival. Homolog Mutant Function
- Lamichhane G et al. [2003]. A postgenomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Mutant
- Mawuenyega KG et al. [2005]. Mycobacterium tuberculosis functional network analysis by global subcellular protein profiling. Proteomics
- Målen H et al. [2010]. Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv. Proteomics
- Griffin JE et al. [2011]. High-resolution phenotypic profiling defines genes essential for mycobacterial growth and cholesterol catabolism. Mutant
- de Souza GA et al. [2011]. Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway. Proteomics
- DeJesus MA et al. [2017]. Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis. Mutant
- Minato Y et al. [2019]. Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways. Mutant
